Optical Quantification by Nanopores of Viruses, Extracellular Vesicles, and Nanoparticles

被引:7
作者
Chazot-Franguiadakis, Lea [1 ]
Eid, Joelle [2 ]
Socol, Marius [2 ]
Molcrette, Bastien [1 ]
Guegan, Philippe [3 ]
Mougel, Marylene [2 ]
Salvetti, Anna [4 ]
Montel, Fabien [1 ]
机构
[1] Univ Lyon, Lab Phys, ENS Lyon, UMR CNRS 5672, F-69007 Lyon, France
[2] Univ Montpellier, Inst Rech Infectiol Montpellier, UMR CNRS 9004, F-34965 Montpellier, France
[3] Sorbonne Univ, Inst Parisien Chim Mol, UMR CNRS 8232, F-75252 Paris, France
[4] Univ Lyon, Ctr Int Rech Infectiol, INSERM, UMR CNRS 5308, F-69007 Lyon, France
关键词
Nanopore; Virus; Extracellular Vesicles; Nanoparticles; Zero Mode Waveguide; Concentration measurement; MODE WAVE-GUIDES; PARTICLES;
D O I
10.1021/acs.nanolett.2c00253
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Nanopores combined with optical approaches canbe used to detect viral particles. In this work, we demonstrate theability of hydrodynamical driving and optical sensing to identifyand quantify viral particles in a biological sample. We havedeveloped a simple and rapid method which requires onlyfluorescent labeling of the particles and can therefore be appliedto a wide range of virus type. The system operates in real time andat the single particle level while providing a low error onconcentration (4%) and a low limit of detection of 105particles/mL for an acquisition time of 60 s with the ability to increase the acquisition time to achieve a lower limit
引用
收藏
页码:3651 / 3658
页数:8
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