Optical Quantification by Nanopores of Viruses, Extracellular Vesicles, and Nanoparticles

被引：7

作者：

Chazot-Franguiadakis, Lea ^{[1
]}

Eid, Joelle ^{[2
]}

Socol, Marius ^{[2
]}

Molcrette, Bastien ^{[1
]}

Guegan, Philippe ^{[3
]}

Mougel, Marylene ^{[2
]}

Salvetti, Anna ^{[4
]}

Montel, Fabien ^{[1
]}

机构：

[1] Univ Lyon, Lab Phys, ENS Lyon, UMR CNRS 5672, F-69007 Lyon, France

[2] Univ Montpellier, Inst Rech Infectiol Montpellier, UMR CNRS 9004, F-34965 Montpellier, France

[3] Sorbonne Univ, Inst Parisien Chim Mol, UMR CNRS 8232, F-75252 Paris, France

[4] Univ Lyon, Ctr Int Rech Infectiol, INSERM, UMR CNRS 5308, F-69007 Lyon, France

来源：

NANO LETTERS | 2022年 / 22卷 / 09期

关键词：

Nanopore; Virus; Extracellular Vesicles; Nanoparticles; Zero Mode Waveguide; Concentration measurement; MODE WAVE-GUIDES; PARTICLES;

D O I：

10.1021/acs.nanolett.2c00253

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nanopores combined with optical approaches canbe used to detect viral particles. In this work, we demonstrate theability of hydrodynamical driving and optical sensing to identifyand quantify viral particles in a biological sample. We havedeveloped a simple and rapid method which requires onlyfluorescent labeling of the particles and can therefore be appliedto a wide range of virus type. The system operates in real time andat the single particle level while providing a low error onconcentration (4%) and a low limit of detection of 105particles/mL for an acquisition time of 60 s with the ability to increase the acquisition time to achieve a lower limit

引用

页码：3651 / 3658

页数：8

共 46 条

[11] Advances in Continuous Microfluidics-Based Technologies for the Study of HIV Infection [J].

Eid, Joelle ;

Mougel, Marylene ;

Socol, Marius .

VIRUSES-BASEL, 2020, 12 (09)

[12]

Farhangdoust F., 2022, ADV MATER, V34

[13] The necessity of and strategies for improving confidence in the accuracy of western blots [J].

Ghosh, Rajeshwary ;

Gilda, Jennifer E. ;

Gomes, Aldrin V. .

EXPERT REVIEW OF PROTEOMICS, 2014, 11 (05) :549-560

[14] The Role of Extracellular Vesicles as Allies of HIV, HCV and SARS Viruses [J].

Giannessi, Flavia ;

Aiello, Alessandra ;

Franchi, Francesca ;

Percario, Zulema Antonia ;

Affabris, Elisabetta .

VIRUSES-BASEL, 2020, 12 (05)

[15] SARS-CoV-2 in river water: Implications in low sanitation countries [J].

Guerrero-Latorre, Laura ;

Ballesteros, Isabel ;

Villacres-Granda, Irina ;

Genoveva Granda, M. ;

Freire-Paspuel, Byron ;

Rios-Touma, Blanca .

SCIENCE OF THE TOTAL ENVIRONMENT, 2020, 743 (743)

[16] Monitoring Assembly of Virus Capsids with Nanofluidic Devices [J].

Harms, Zachary D. ;

Selzer, Lisa ;

Zlotnick, Adam ;

Jacobson, Stephen C. .

ACS NANO, 2015, 9 (09) :9087-9096

[17] Single-Particle Electrophoresis in Nanochannels [J].

Harms, Zachary D. ;

Haywood, Daniel G. ;

Kneller, Andrew R. ;

Selzer, Lisa ;

Zlotnick, Adam ;

Jacobson, Stephen C. .

ANALYTICAL CHEMISTRY, 2015, 87 (01) :699-705

[18] Broad-Spectrum Inhibition of Retroviral and Filoviral Particle Release by Tetherin [J].

Jouvenet, Nolwenn ;

Neil, Stuart J. D. ;

Zhadina, Maria ;

Zang, Trinity ;

Kratovac, Zerina ;

Lee, Youngnam ;

McNatt, Matthew ;

Hatziioannou, Theodora ;

Bieniasz, Paul D. .

JOURNAL OF VIROLOGY, 2009, 83 (04) :1837-1844

[19] Fluids at the Nanoscale: From Continuum to Subcontinuum Transport [J].

Kavokine, Nikita ;

Netz, Roland R. ;

Bocquet, Lyderic .

ANNUAL REVIEW OF FLUID MECHANICS, VOL 53, 2021, 53 :377-410

[20] Palladium zero-mode waveguides for optical single-molecule detection with nanopores [J].

Klughammer, Nils ;

Dekker, Cees .

NANOTECHNOLOGY, 2021, 32 (18)

← 1 2 3 4 5 →