Transplantation of UC-MSCs on collagen scaffold activates follicles in dormant ovaries of POF patients with long history of infertility

被引：148

作者：

Ding, Lijun ^{[1
,2
]}

Yan, Guijun ^{[1
]}

Wang, Bin ^{[1
,2
]}

Xu, Lu ^{[1
]}

Gu, Yan ^{[3
]}

Ru, Tong ^{[3
]}

Cui, Xiaoying ^{[1
]}

Lei, Lei ^{[1
]}

Liu, Jingyu ^{[1
]}

Sheng, Xiaoqiang ^{[1
]}

Wang, Bin ^{[1
,2
]}

Zhang, Chunxue ^{[1
]}

Yang, Yanjun ^{[1
]}

Jiang, Ruiwei ^{[1
]}

Zhou, Jianjun ^{[1
]}

Kong, Na ^{[1
]}

Lu, Feifei ^{[1
]}

Zhou, Huaijun ^{[3
]}

Zhao, Yannan ^{[4
]}

Chen, Bing ^{[4
]}

Hu, Yali ^{[1
]}

Dai, Jianwu ^{[4
]}

Sun, Haixiang ^{[1
]}

机构：

[1] Nanjing Univ, Affiliated Drum Tower Hosp, Dept Obstet & Gynecol, Ctr Reprod Med,Med Sch, Nanjing 210008, Jiangsu, Peoples R China

[2] Nanjing Univ, Affiliated Drum Tower Hosp, Clin Ctr Stem Cell Res, Med Sch, Nanjing 210008, Jiangsu, Peoples R China

[3] Nanjing Univ, Affiliated Drum Tower Hosp, Dept Obstet & Gynecol, Med Sch, Nanjing 210008, Jiangsu, Peoples R China

[4] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100190, Peoples R China

来源：

SCIENCE CHINA-LIFE SCIENCES | 2018年 / 61卷 / 12期

基金：

中国国家自然科学基金;

关键词：

premature ovarian failure; collagen scaffold; UC-MSCs; primordial follicle activation; granulosa cells; FOXO3a; FOXO1; IN-VITRO ACTIVATION; STEM-CELLS; KIT-LIGAND; RAT MODEL; EXPRESSION; FERTILITY; PATHWAY; FOXO3;

D O I：

10.1007/s11427-017-9272-2

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Premature ovarian failure (POF) is a refractory disease for clinical treatment with the goal of restoring fertility. In this study, umbilical cord mesenchymal stem cells on a collagen scaffold (collagen/UC-MSCs) can activate primordial follicles in vitro via phosphorylation of FOXO3a and FOXO1. Transplantation of collagen/UC-MSCs to the ovaries of POF patients rescued overall ovarian function, evidenced by elevated estradiol concentrations, improved follicular development, and increased number of antral follicles. Successful clinical pregnancy was achieved in women with POF after transplantation of collagen/UC-MSCs or UC-MSCs. In summary, collagen/UC-MSC transplantation may provide an effective treatment for POF.

引用

页码：1554 / 1565

页数：12

共 35 条

[1] Dysregulated FOXO transcription factors in articular cartilage in aging and osteoarthritis [J].

Akasaki, Y. ;

Hasegawa, A. ;

Saito, M. ;

Asahara, H. ;

Iwamoto, Y. ;

Lotz, M. K. .

OSTEOARTHRITIS AND CARTILAGE, 2014, 22 (01) :162-170

[2] FoxO3a eggs on fertility and aging [J].

Brenkman, AB ;

Burgering, BMT .

TRENDS IN MOLECULAR MEDICINE, 2003, 9 (11) :464-467

[3] The PTEN-PI3K pathway: of feedbacks and cross-talks [J].

Carracedo, A. ;

Pandolfi, P. P. .

ONCOGENE, 2008, 27 (41) :5527-5541

[4] Suppression of ovarian follicle activation in mice by the transcription factor Foxo3a [J].

Castrillon, DH ;

Miao, LL ;

Kollipara, R ;

Horner, JW ;

DePinho, RA .

SCIENCE, 2003, 301 (5630) :215-218

[5] Effect of cryopreservation and transplantation on the expression of kit ligand and anti-Mllerian hormone in human ovarian tissue [J].

David, Anu ;

Van Langendonckt, Anne ;

Gilliaux, Sebastien ;

Dolmans, Marie-Madeleine ;

Donnez, Jacques ;

Amorim, Christiani A. .

HUMAN REPRODUCTION, 2012, 27 (04) :1088-1095

[6] Primary ovarian insufficiency [J].

De Vos, Michel ;

Devroey, Paul ;

Fauser, Bart C. J. M. .

LANCET, 2010, 376 (9744) :911-921

[7] Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects [J].

Elfayomy, Amr K. ;

Almasry, Shaima M. ;

El-Tarhouny, Shereen A. ;

Eldomiaty, Magda A. .

TISSUE & CELL, 2016, 48 (04) :370-382

[8] Kit ligand and glial-derived neurotrophic factor as alternative supplements for activation and development of ovine preantral follicles in vitro [J].

Esmaielzadeh, F. ;

Hosseini, S. M. ;

Nasiri, Z. ;

Hajian, M. ;

Chamani, M. ;

Gourabi, H. ;

Shahverdi, A. H. ;

Vosough, A. D. ;

Nasr-Esfahani, M. H. .

MOLECULAR REPRODUCTION AND DEVELOPMENT, 2013, 80 (01) :35-47

[9] Regulation of FOXO3 subcellular localization by Kit ligand in the neonatal mouse ovary [J].

Ezzati, M. Max ;

Baker, Michael D. ;

Saatcioglu, Hatice D. ;

Aloisio, Gina M. ;

Pena, Christopher G. ;

Nakada, Yuji ;

Cuevas, Ileana ;

Carr, Bruce R. ;

Castrillon, Diego H. .

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 2015, 32 (12) :1741-1747

[10] Transplantation of human mesenchymal stem cells loaded on collagen scaffolds for the treatment of traumatic brain injury in rats [J].

Guan, Jian ;

Zhu, Zhaohui ;

Zhao, Robert Chunhua ;

Xiao, Zhifeng ;

Wu, Chenxi ;

Han, Qin ;

Chen, Lei ;

Tong, Weimin ;

Zhang, Jin ;

Han, Qianqian ;

Gao, Jun ;

Feng, Ming ;

Bao, Xinjie ;

Dai, Jianwu ;

Wang, Renzhi .

BIOMATERIALS, 2013, 34 (24) :5937-5946

← 1 2 3 4 →