MicroRNA 1301 inhibits cisplatin resistance in human ovarian cancer cells by regulating EMT and autophagy
被引:2
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作者:
Yu, J-L
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机构:
Chifeng City Hosp, Dept Gynecol, Chifeng City, Inner Mongolia, Peoples R ChinaChifeng City Hosp, Dept Gynecol, Chifeng City, Inner Mongolia, Peoples R China
Yu, J-L
[1
]
Gao, X.
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机构:
Chifeng City Hosp, Dept Gynecol, Chifeng City, Inner Mongolia, Peoples R ChinaChifeng City Hosp, Dept Gynecol, Chifeng City, Inner Mongolia, Peoples R China
Gao, X.
[1
]
机构:
[1] Chifeng City Hosp, Dept Gynecol, Chifeng City, Inner Mongolia, Peoples R China
OBJECTIVE: Ovarian cancer is prone to chemoresistance. leading to poor out-comes in patients. MicroRNA 1301 plays a regulatory role in multiple tumors. However, whether microRNA 1301 regulates cisplatin resistance in ovarian cancer cells remains unclear. MATERIALS AND METHODS: The ovarian cancer SKOV3 cell line and the human ovarian cancer cisplatin-resistant strain cell SKOV3/ DDP were cultured in vitro and microRNA1301 expression was analyzed by Real time PCR. MicroRNA1301 mimics and microRNA 1301 were transfected into SKOV3/DDP. respectively fol-lowed by analysis of cell proliferation by MTT assay, cell invasion, expression of autophagy genes ATG5 and Beclin1 and EMT-related transcription factors Snail and Slug by Real time PCR, expression of NF-kappa B and E-cadherin and N-cadherin by Western blot. RESULTS: MicroRNA 1301 expression was significantly increased in SKOV3/DDP cells compared with that in SKOV3 cells (p<0.05). MicroR-NA1301 mimics transfection into SKOV3/DDP up-regulated microRNA1301 expression, promoted cell proliferation, and invasion, inhibit-ed ATG5 and Beclinl expression, and promoted Snail and Slug expression, decreased E-cadherin expression and increased N-cadherin and NF-kappa B expression, compared with the control group, the differences were statistically significant (p<0.05). MicroRNA1301 inhibitor transfection into SKOV3/DDP cells could down-regulate the expression of microRNA1301 and significantly reversed the above changes. Compared with the control group, differences were statistically significant (p<0.05). CONCLUSIONS: Targeting microRNA1301 can inhibit the proliferation of cisplatin-resistant cells and the development of EMT in human ovarian cancer cells by inhibiting the NF-kappa B signaling pathway, thereby inhibiting the occurrence and development of drug-resistant ovarian cancer.
机构:
Hosp Chengdu Univ Tradit Chinese Med, Dept Oncol, Chengdu, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Dept Oncol, Chengdu, Peoples R China
Cai, Yi
An, Baiping
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Hosp Chengdu Univ Tradit Chinese Med, Dept Oncol, Chengdu, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Dept Oncol, Chengdu, Peoples R China
An, Baiping
Yao, Dejiao
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Hosp Chengdu Univ Tradit Chinese Med, Dept Oncol, Chengdu, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Dept Oncol, Chengdu, Peoples R China
Yao, Dejiao
Zhou, Hong
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Hosp Chengdu Univ Tradit Chinese Med, Dept Oncol, Chengdu, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Dept Oncol, Chengdu, Peoples R China
Zhou, Hong
Zhu, Jie
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Hosp Chengdu Univ Tradit Chinese Med, Dept Oncol, Chengdu, Peoples R ChinaHosp Chengdu Univ Tradit Chinese Med, Dept Oncol, Chengdu, Peoples R China
机构:Wayne State Univ Sch Med, Karmanos Canc Inst, Dept Oncol, Detroit, MI 48201 USA
Wang, Juan
Wu, Gen Sheng
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机构:
Wayne State Univ Sch Med, Karmanos Canc Inst, Dept Oncol, Detroit, MI 48201 USAWayne State Univ Sch Med, Karmanos Canc Inst, Dept Oncol, Detroit, MI 48201 USA
机构:
Chinese Acad Med Sci, Inst Canc, Lab Cell & Mol Biol, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Inst Canc, State Key Lab Mol Oncol, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Canc Hosp, Beijing 100021, Peoples R China
Peking Union Med Coll, Beijing 100021, Peoples R ChinaChinese Acad Med Sci, Inst Canc, Lab Cell & Mol Biol, Beijing 100021, Peoples R China
Liu, Mei
Zhang, Xin
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机构:
China Meitan Gen Hosp, Dept Obstet & Gynecol, Beijing 100021, Peoples R ChinaChinese Acad Med Sci, Inst Canc, Lab Cell & Mol Biol, Beijing 100021, Peoples R China
Zhang, Xin
Hu, Chen-Fei
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Chinese Acad Med Sci, Inst Canc, Lab Cell & Mol Biol, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Inst Canc, State Key Lab Mol Oncol, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Canc Hosp, Beijing 100021, Peoples R China
Peking Union Med Coll, Beijing 100021, Peoples R ChinaChinese Acad Med Sci, Inst Canc, Lab Cell & Mol Biol, Beijing 100021, Peoples R China
Hu, Chen-Fei
Xu, Qing
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机构:
Chinese Acad Med Sci, Inst Canc, Lab Cell & Mol Biol, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Inst Canc, State Key Lab Mol Oncol, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Canc Hosp, Beijing 100021, Peoples R China
Peking Union Med Coll, Beijing 100021, Peoples R ChinaChinese Acad Med Sci, Inst Canc, Lab Cell & Mol Biol, Beijing 100021, Peoples R China
Xu, Qing
Zhu, Hong-Xia
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Chinese Acad Med Sci, Inst Canc, Lab Cell & Mol Biol, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Inst Canc, State Key Lab Mol Oncol, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Canc Hosp, Beijing 100021, Peoples R China
Peking Union Med Coll, Beijing 100021, Peoples R ChinaChinese Acad Med Sci, Inst Canc, Lab Cell & Mol Biol, Beijing 100021, Peoples R China
Zhu, Hong-Xia
Xu, Ning-Zhi
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Chinese Acad Med Sci, Inst Canc, Lab Cell & Mol Biol, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Inst Canc, State Key Lab Mol Oncol, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Canc Hosp, Beijing 100021, Peoples R China
Peking Union Med Coll, Beijing 100021, Peoples R ChinaChinese Acad Med Sci, Inst Canc, Lab Cell & Mol Biol, Beijing 100021, Peoples R China
机构:
Shanxi Prov Peoples Hosp, Gynaecol & Obstet, Taiyuan 030012, Shanxi, Peoples R ChinaShanxi Prov Peoples Hosp, Gynaecol & Obstet, Taiyuan 030012, Shanxi, Peoples R China
Li, Hui
Lei, Yuansheng
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机构:
Shanxi Med Univ, Hosp 2, Internal Med Neurol, Taiyuan 030001, Shanxi, Peoples R ChinaShanxi Prov Peoples Hosp, Gynaecol & Obstet, Taiyuan 030012, Shanxi, Peoples R China
Lei, Yuansheng
Li, Shuangxue
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机构:
Shanxi Prov Peoples Hosp, Resp Med, Taiyuan 030012, Shanxi, Peoples R ChinaShanxi Prov Peoples Hosp, Gynaecol & Obstet, Taiyuan 030012, Shanxi, Peoples R China
Li, Shuangxue
Li, Feng
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机构:
Shanxi Med Univ, Gen Hosp Taigang, Dept Gen Surg, Taiyuan 030003, Shanxi, Peoples R ChinaShanxi Prov Peoples Hosp, Gynaecol & Obstet, Taiyuan 030012, Shanxi, Peoples R China
Li, Feng
Lei, Jieyun
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机构:
Shanxi Med Univ, Taiyuan Cent Hosp, Gynaecol & Obstet, Bldg 3,Wuyi Rd, Taiyuan 030010, Shanxi, Peoples R ChinaShanxi Prov Peoples Hosp, Gynaecol & Obstet, Taiyuan 030012, Shanxi, Peoples R China
机构:
Chinese Peoples Liberat Army, Dept Gastroenterol, Gen Hosp, Beijing, Peoples R ChinaChinese Peoples Liberat Army, Dept Gastroenterol, Gen Hosp, Beijing, Peoples R China
Li, Meng
Zhang, Hong-Yi
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机构:
Capital Med Univ, Beijing Elect Power Hosp, Dept Stomatol, Beijing, Peoples R ChinaChinese Peoples Liberat Army, Dept Gastroenterol, Gen Hosp, Beijing, Peoples R China
Zhang, Hong-Yi
Zhang, Rong-Gui
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机构:
Chinese Peoples Liberat Army, Dept Gastroenterol, Gen Hosp, Beijing, Peoples R ChinaChinese Peoples Liberat Army, Dept Gastroenterol, Gen Hosp, Beijing, Peoples R China