Papain immobilization on a nitrilon fibre carrier containing primary amine groups

被引：28

作者：

Li, YF ^{[1
]}

Jia, FY

Li, JR

Liu, G

Li, YZ

机构：

[1] Lanzhou Univ, Inst Mat Sci & Engn, Dept Chem, Lanzhou 730000, Peoples R China

[2] Gansu Prov Inst Drug Control, Lanzhou 730000, Peoples R China

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2001年 / 33卷

关键词：

enzymic reaction; proteolytic enzyme;

D O I：

10.1042/BA19990015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A carrier containing primary amine groups was synthesized by the reaction between nitrilon fibre and diethylenetriamine, and papain was immobilized on this kind of carrier by using glutaraldehyde as a coupling agent. The factors involved with the activity recovery of the immobilized papain and the enzymic properties of the resulting immobilized papain were studied in comparison with free papain, for which casein was chosen as a substrate. The results show that the activity recovery of immobilized papain can reach 47.3%, and that the immobilized papain exhibits better environmental adaptability and reusability than free papain.

引用

页码：29 / 34

页数：6

共 15 条

[1]

ATKINSON B, 1985, BIOCH ENG BIOTECHNOL, P508

[2] ANTIGENS AND ENZYMES MADE INSOLUBLE BY ENTRAPPING THEM INTO LATTICES OF SYNTHETIC POLYMERS [J].

BERNFELD, P ;

WAN, J .

SCIENCE, 1963, 142 (359) :678-+

[3] Biofunctional membranes: An EPR study of active site structure and stability of papain non-covalently immobilized on the surface of modified poly(ether) sulfone membranes through the avidin-biotin linkage [J].

Bhardwaj, A ;

Lee, JB ;

Glauner, K ;

Ganapathi, S ;

Bhattacharyya, D ;

Butterfield, DA .

JOURNAL OF MEMBRANE SCIENCE, 1996, 119 (02) :241-252

[4] Synthesis of poly(N-aminoethyl)acrylamide chelating fiber and properties of concentration and separation of noble metal ions from samples [J].

Chang, XJ ;

Li, YF ;

Zhan, GY ;

Luo, XY ;

Gao, WY .

TALANTA, 1996, 43 (03) :407-413

[5] PAPAIN IMMOBILIZATION ONTO POROUS POLY(GAMMA-METHYL L-GLUTAMATE) BEADS [J].

HAYASHI, T ;

HIRAYAMA, C ;

IWATSUKI, M .

JOURNAL OF APPLIED POLYMER SCIENCE, 1992, 44 (01) :143-150

[6]

HE F, 1997, ION EXCHANGE ADSORPT, V13, P290

[7] Peptide synthesis by papain in alkali halide media [J].

Lozano, P ;

Avellaneda, A ;

Iborra, JL .

BIOCATALYSIS AND BIOTRANSFORMATION, 1996, 13 (04) :255-269

[8]

MUZZARELLI RAA, 1985, POLYSACCHARIDES, V3, P417

[9]

PERLMANN GE, 1970, METHOD ENZYMOL, V19, P226

[10] Dehydrooligopeptides .18. Enzymatic hydrolysis and coupling of dehydrodipeptide esters containing alpha-dehydroamino acid residue by using papain [J].

Shin, CG ;

Kakusho, T ;

Arai, K ;

Seki, M .

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 1995, 68 (12) :3549-3555

← 1 2 →