Graphene-Based SELDI Probe with Ultrahigh Extraction and Sensitivity for DNA Oligomer

被引：251
作者：
Tang, Lena Ai Ling [1 ]
Wang, Junzhong [1 ]
Loh, Kian Ping [1 ]
机构：
[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 32期
关键词：
NANOPARTICLES; OXIDE;
D O I：
10.1021/ja104017y
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We demonstrate that graphene can be used as an ultrahigh efficiency preconcentration and detection platform for ssDNA. DNA-adsorbed graphene can be used directly for Surface Enhanced Laser Desorption Ionization-Time of Flight-Mass Spectrometry (SELDI-TOF-MS). The rapid enrichment of biomolecules and direct, label-free detection are potentially useful for analysis in proteomics and genomics.
引用
收藏
页码：10976 / 10977
页数：2
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  • [1] Superior thermal conductivity of single-layer graphene
    Balandin, Alexander A.
    Ghosh, Suchismita
    Bao, Wenzhong
    Calizo, Irene
    Teweldebrhan, Desalegne
    Miao, Feng
    Lau, Chun Ning
    [J]. NANO LETTERS, 2008, 8 (03) : 902 - 907
  • [2] Graphene: Status and Prospects
    Geim, A. K.
    [J]. SCIENCE, 2009, 324 (5934) : 1530 - 1534
  • [3] PREPARATION OF GRAPHITIC OXIDE
    HUMMERS, WS
    OFFEMAN, RE
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1958, 80 (06) : 1339 - 1339
  • [4] DNA detection using a triple readout optical/AFM/MALDI planar microwell plastic chip
    Ibanez, Alfredo J.
    Schueler, Thomas
    Moeller, Robert
    Fritzsche, Wolfgang
    Saluz, Hans-Peter
    Svatos, Ales
    [J]. ANALYTICAL CHEMISTRY, 2008, 80 (15) : 5892 - 5898
  • [5] Polylysine-coated diamond nanocrystals for MALDI-TOF mass analysis of DNA oligonucleotides
    Kong, XL
    Huang, LCL
    Liau, SCV
    Han, CC
    Chang, HC
    [J]. ANALYTICAL CHEMISTRY, 2005, 77 (13) : 4273 - 4277
  • [6] High-affinity capture of proteins by diamond nanoparticles for mass spectrometric analysis
    Kong, XL
    Huang, LCL
    Hsu, CM
    Chen, WH
    Han, CC
    Chang, HC
    [J]. ANALYTICAL CHEMISTRY, 2005, 77 (01) : 259 - 265
  • [7] PEGylated nanographene oxide for delivery of water-insoluble cancer drugs
    Liu, Zhuang
    Robinson, Joshua T.
    Sun, Xiaoming
    Dai, Hongjie
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (33) : 10876 - +
  • [8] Conformation of oligodeoxynucleotides associated with anionic liposomes
    Patil, SD
    Rhodes, DG
    [J]. NUCLEIC ACIDS RESEARCH, 2000, 28 (21) : 4125 - 4129
  • [9] Cytochrome c on Silica Nanoparticles: Influence of Nanoparticle Size on Protein Structure, Stability, and Activity
    Shang, Wen
    Nuffer, Joseph H.
    Muniz-Papandrea, Virginio A.
    Colon, Wilfredo
    Siegel, Richard W.
    Dordick, Jonathan S.
    [J]. SMALL, 2009, 5 (04) : 470 - 476
  • [10] Ion Desorption Efficiency and Internal Energy Transfer in Carbon-Based Surface-Assisted Laser Desorption/Ionization Mass Spectrometry: Desorption Mechanism(s) and the Design of SALDI Substrates
    Tang, Ho-Wai
    Ng, Kwan-Ming
    Lu, Wei
    Che, Chi-Ming
    [J]. ANALYTICAL CHEMISTRY, 2009, 81 (12) : 4720 - 4729
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