Neuroactive steroids protect retinal pigment epithelium against oxidative stress

被引：20
|
作者：
Bucolo, C [1 ]
Drago, F
Lin, LR
Reddy, VN
机构：
[1] Univ Catania, Sch Med, Dept Expt & Clin Pharmacol, I-95125 Catania, Italy
[2] Univ Michigan, Kellogg Eye Ctr, Dept Ophthalmol & Visual Sci, Ann Arbor, MI 48109 USA
来源：
NEUROREPORT | 2005年 / 16卷 / 11期
关键词：
neuroactive steroids; oxidative stress; retinal pigment epithelial cells; sigma(1)receptors;
D O I：
10.1097/00001756-200508010-00014
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
This study was undertaken to assess whether neuroactive steroids, 17 beta-estradiol and dehydroepiandrosterone-sulfate, enhance survival and protect DNA of human retinal pigment epithelial cells challenged by oxidative stress, and to investigate the role of sigma(1) receptors in the effects of neuroactive steroids. Retinal pigment epithelial cells were treated with various concentrations of neuroactive steroids and then exposed to hydrogen peroxide. Pretreatment with steroids resulted in significant increased viability in a dose-related manner. DNA damage induced by oxidative insult was significantly lower with steroid pretreatment. The effects of 17 beta-estradiol and dehydroepiandrosterone-sulfate were antagonized by pretreatment with a a, receptor antagonist. The results suggest that neuroactive steroids protect retinal cells from oxidative stress, and that this effect is mediated by sigma(1) receptors.
引用
收藏
页码：1203 / 1207
页数：5
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