Real-Time Monitoring of Solid-Phase PCR Using Fiber-Optic SPR

被引：29
|
作者：
Pollet, Jeroen [1 ]
Janssen, Kris P. F. [1 ]
Knez, Karel [1 ]
Lammertyn, Jeroen [1 ]
机构：
[1] Katholieke Univ Leuven, BIOSYST MeBioS, B-3001 Heverlee, Belgium
来源：
SMALL | 2011年 / 7卷 / 08期
关键词：
SURFACE-PLASMON RESONANCE; POLYMERASE-CHAIN-REACTION; FUNCTIONALIZED GOLD NANOPARTICLES; ENZYMATIC AMPLIFICATION; SIGNAL AMPLIFICATION; DNA INVITRO; HYBRIDIZATION; IMMUNOASSAY; ANTIBODY;
D O I：
10.1002/smll.201001984
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Real-time monitoring of solid-phase DNA amplification using a surface plasmon resonance sensor (SPR) with gold nanobead signal enhancement is presented. The sensor is applied in the quantification of a DNA aptamer and subsequent melting curve analysis. The system is suited for application in medical and food-safety diagnostics, and for the study and optimization of solid-phase polymerase chain reaction. © 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：1003 / 1006
页数：4
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