A rapid, automated VWF ristocetin cofactor activity assay improves reliability in the diagnosis of Von Willebrand disease

被引:13
作者
Bowyer, Annette E. [1 ,2 ]
Shepherd, Fiona [1 ]
Kitchen, Stephen [1 ]
Makris, Michael [1 ,2 ]
机构
[1] Royal Hallamshire Hosp, Sheffield Haemophilia & Thrombosis Ctr, Sheffield S10 2JF, S Yorkshire, England
[2] Univ Sheffield, Dept Cardiovasc Sci, Sheffield S10 2TN, S Yorkshire, England
关键词
VWF:RCo; Von Willebrand disease; Ristocetin; PREVALENCE;
D O I
10.1016/j.thromres.2010.11.029
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Introduction: The effective diagnosis and monitoring of Von Willebrand Disease (VWD) requires an accurate assessment of ristocetin co-factor activity (VWF:RCo). Current methodologies include automated platelet aggregometry and manual visual agglutination both of which are laborious to perform and notoriously subject to a high degree of inter and intra assay variation. Methods and Materials: We have evaluated an automated VWF: RCo assay (BC Von Willebrand Reagent, Siemens, Marberg, Germany) for use on the Sysmex CS2100i analyser (Milton Keynes, UK) and retrospectively compared the results with an in-house manual visual agglutination assay and VWF antigen (Siemens) in normal subjects and in 53 patients with various types of VWD and 23 patients following VWF therapeutic treatment. Results: The intra and interassay CV was improved with the automated assay (2.3% and 3.8% respectively) compared to 7% with the manual VWF: RCo assay. Good correlation was found between the two assays (r = 0.91) in 53 patients with VWD. The mean manual VWF: RCo was 0.25 IU/ml and mean automated VWF: RCo was 0.27 IU/ml. A comparable increase in VWF: RCo following treatment, mostly with Desmopressin, was found in 13 patients with type 1 VWD (mean 3.9 fold increase with manual VWF: RCo and 3.1 fold with the automated VWF: RCo). In 13 patients with type 2 or 3 VWD following treatment mostly with concentrate, a higher increase was found with the automated VWF: RCo assay than the manual assay (mean 11.9 fold manually and mean 20.3 automated). Conclusion: The automated VWF: RCo assay shows enhanced precision and analysis time in this difficult and time consuming laboratory test and its introduction should greatly improve the reliability of VWF testing. (C) 2010 Published by Elsevier Ltd.
引用
收藏
页码:341 / 344
页数:4
相关论文
共 14 条
[1]   ASSAY OF WILLEBRAND FACTOR IN VONWILLEBRANDS DISEASE AND HEMOPHILIA - USE OF A MACROSCOPIC PLATELET-AGGREGATION TEST [J].
BRINKHOUS, KM ;
GRAHAM, JE ;
COOPER, HA ;
ALLAIN, JP ;
WAGNER, RH .
THROMBOSIS RESEARCH, 1975, 6 (03) :267-272
[2]  
Casonato A, 1999, THROMB HAEMOSTASIS, V81, P994
[3]   ASSAY OF RISTOCETIN COFACTOR USING FIXED PLATELETS AND A PLATELET COUNTING TECHNIQUE [J].
EVANS, RJ ;
AUSTEN, DEG .
BRITISH JOURNAL OF HAEMATOLOGY, 1977, 37 (02) :289-294
[4]   Laboratory diagnosis of von Willebrand disorder - Current practice in the Southern Hemisphere [J].
Favaloro, EJ ;
Bonar, R ;
Sioufi, J ;
Hertzberg, M ;
Street, A ;
Lloyd, J ;
Marsden, K .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2003, 119 (06) :882-893
[5]   External peer review quality assurance testing in von Willebrand disease: The recent experience of the United States College of American Pathologists proficiency testing program [J].
Hayes, Timothy E. ;
Brandt, John T. ;
Chandler, Wayne L. ;
Eby, Charles S. ;
Kottke-Marchant, Kandice ;
Krishnan, Jayashree ;
Lefkowitz, Jerry B. ;
Olson, John D. ;
Rund, Chad R. ;
Van Cott, Elizabeth M. ;
Cunningham, Mark T. .
SEMINARS IN THROMBOSIS AND HEMOSTASIS, 2006, 32 (05) :499-504
[6]   Laboratory tests for measurement of von Willebrand factor show poor agreement among different centers: Results from the United Kingdom National External Quality Assessment Scheme for Blood Coagulation [J].
Kitchen, Steve ;
Jennings, Ion ;
Woods, Tim A. L. ;
Kitchen, Dianne P. ;
Walker, Isobel D. ;
Preston, Francis E. .
SEMINARS IN THROMBOSIS AND HEMOSTASIS, 2006, 32 (05) :492-498
[7]   A comparative in vitro evaluation of six von Willebrand factor concentrates [J].
Lethagen, S ;
Carlson, M ;
Hillarp, A .
HAEMOPHILIA, 2004, 10 (03) :243-249
[8]   Drug therapy - Treatment of von Willebrand's disease [J].
Mannucci, PM .
NEW ENGLAND JOURNAL OF MEDICINE, 2004, 351 (07) :683-694
[9]   An external quality assessment program for von Willebrand factor laboratory analysis: An overview from the European Concerted Action on Thrombosis and Disabilities Foundation [J].
Meijer, Piet ;
Haverkate, Frits .
SEMINARS IN THROMBOSIS AND HEMOSTASIS, 2006, 32 (05) :485-491
[10]  
Miller CH, 2002, THROMB HAEMOSTASIS, V88, P56