Encapsulation of enzymes in microgels by polymerization/cross-linking in aqueous droplets

被引:60
作者
Schachschal, Susann [2 ]
Adler, Hans-Juergen [2 ]
Pich, Andrij [1 ,2 ]
Wetzel, Stefanie
Matura, Anke
van Pee, Karl-Heinz
机构
[1] DWI RWTH Aachen Univ, D-52056 Aachen, Germany
[2] Tech Univ Dresden, Inst Macromol Chem, D-01062 Dresden, Germany
关键词
Microgel; Enzyme; Encapsulation; TRAMETES-VERSICOLOR; ALPHA-CHYMOTRYPSIN; N-ISOPROPYLACRYLAMIDE; IMMOBILIZATION; LACCASE; PARTICLES; HYDROGEL; CARRIERS; BEADS; DECOLORIZATION;
D O I
10.1007/s00396-011-2392-1
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
In the present paper, we describe the synthesis of poly(N-isopropylacrylamide) microgel particles in water/oil inverse emulsion used as carriers for enzyme encapsulation. Since enzymes can be used in different biotechnological applications, their immobilization on polymer colloidal carriers is of great importance. In this work, laccase from Trametes versicolor and also several peroxidases were immobilized in microgels. The polymerization process occurred via radical initiation in aqueous droplets in the presence of the comonomer vinylimidazole and the cross-linker N',N'-methylene-bis-acrylamide at room temperature. Non-ionic surfactants were used to stabilize aqueous droplets in heptane. Due to the formation of microgels in aqueous media and the low reaction temperature, this technique allows the encapsulation of enzymes without risk of their denaturation, which can be controlled by variation of reaction parameters. Enzyme-containing microgels obtained by this method were analyzed in detail concerning their particle size, swelling properties, zeta potential, as well as resulting enzyme activity. Our experimental data indicate that the presence of imidazole groups in microgel structure allows increasing enzyme loading without reduction of enzyme activity.
引用
收藏
页码:693 / 698
页数:6
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