circCAMSAP1 Promotes Tumor Growth in Colorectal Cancer via the miR-328-5p/E2F1 Axis

被引：127

作者：

Zhou, Chi ^{[1
,2
,3
]}

Liu, Hua-shan ^{[1
,2
,3
]}

Wang, Feng-wei ^{[4
]}

Hu, Tuo ^{[1
,2
,3
]}

Liang, Zhen-xing ^{[1
,2
,3
]}

Lan, Nan ^{[1
,2
]}

He, Xiao-wen ^{[1
,2
]}

Zheng, Xiao-bin ^{[1
,2
,3
]}

Wu, Xiao-jian ^{[1
,2
]}

Xie, Dan ^{[4
]}

Wu, Xian-rui ^{[1
,2
,3
]}

Lan, Ping ^{[1
,2
,3
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 6, Dept Colorectal Surg, 26 Yuancun Erheng Rd, Guangzhou 510655, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Affiliated Hosp 6, Guangdong Prov Key Lab Colorectal & Pelv Floor Di, Guangzhou, Guangdong, Peoples R China

[3] Guangzhou Regenerat Med & Hlth Guangdong Lab, Guangzhou, Peoples R China

[4] Sun Yat Sen Univ, Collaborat Innovat Ctr Canc Med, State Key Lab Oncol South China, Canc Ctr, Guangzhou 510060, Peoples R China

来源：

MOLECULAR THERAPY | 2020年 / 28卷 / 03期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

CIRCULAR RNA; EXPRESSION; BIOMARKER;

D O I：

10.1016/j.ymthe.2019.12.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Increasing studies indicated that circular RNAs (circRNAs) play important roles in cancer progression. However, the roles of circRNAs in colorectal cancer (CRC) remain largely unknown. In this study, we determined the circRNA expression profile by next-generation RNA sequencing from eight CRC and paired non-cancerous matched tissues. circCAMSAP1 (originating from exon 2 to exon 3 of the CAMSAP1 gene, hsa_ circ_0001900) was significantly upregulated in CRC tissues. Increased circCAMSAP1 expression was significantly correlated with advanced tumor/node/metastasis (TNM) stage and shortened overall survival. An elevation of circCAMSAP1 expression was detected via droplet digital PCR in the serum of CRC patients prior to surgery. Functionally, circCAMSAP1 promoted the malignant behavior of CRC. Mechanism study of upstream biogenesis of circCAMSAP1 indicated that circCAMSAP1 cyclization inCRC wasmediated by splicing factor epithelial-splicing regulatory protein 1. Moreover, circCAMSAP1 acted as a sponge for miR-328-5p and abrogated its suppression on transcription factor E2F1. Taken together, our data indicated an essential role of the circCAMSAP1/miR-328-5p/E2F1 axis in the progression of CRC, which implied that circCAMSAP1 could serve as a diagnostic and prognostic biomarker as well as a potential therapeutic target for CRC.

引用

页码：914 / 928

页数：15

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