Interactions of the Gasotransmitters Contribute to Microvascular Tone (Dys) regulation in the Preterm Neonate

被引:15
作者
Dyson, Rebecca M. [1 ,2 ,3 ,4 ]
Palliser, Hannah K. [1 ,5 ]
Latter, Joanna L. [1 ,2 ]
Kelly, Megan A. [3 ,4 ]
Chwatko, Grazyna [6 ]
Glowacki, Rafal [6 ]
Wright, Ian M. R. [1 ,2 ,3 ,4 ,7 ]
机构
[1] Hunter Med Res Inst, Mothers & Babies Res Ctr, New Lambton Hts, NSW 2305, Australia
[2] Univ Newcastle, Sch Med & Publ Hlth, Callaghan, NSW 2308, Australia
[3] Univ Wollongong, Illawarra Hlth & Med Res Inst, Wollongong, NSW 2522, Australia
[4] Univ Wollongong, Grad Sch Med, Wollongong, NSW 2522, Australia
[5] Univ Newcastle, Sch Biomed Sci & Pharm, Callaghan, NSW 2308, Australia
[6] Univ Lodz, Fac Chem, Dept Environm Chem, PL-90236 Lodz, Poland
[7] John Hunter Childrens Hosp, Kaleidoscope Neonatal Intens Care Unit, New Lambton Hts, NSW 2305, Australia
来源
PLOS ONE | 2015年 / 10卷 / 03期
基金
英国医学研究理事会;
关键词
NITRIC-OXIDE SYNTHASE; GLUCOCORTICOID-MEDIATED HYPERTENSION; INDUCED CEREBROVASCULAR DILATION; RESPIRATORY-DISTRESS-SYNDROME; CYSTATHIONINE BETA-SYNTHASE; OXYGENASE-1; GENE-EXPRESSION; VASCULAR ENDOTHELIAL-CELLS; CARBON-MONOXIDE PRODUCTION; SMOOTH-MUSCLE RELAXANT; 1A RECEPTOR GENE;
D O I
10.1371/journal.pone.0121621
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background & Aims Hydrogen sulphide (H2S), nitric oxide (NO), and carbon monoxide (CO) are involved in transitional microvascular tone dysregulation in the preterm infant; however there is conflicting evidence on the interaction of these gasotransmitters, and their overall contribution to the microcirculation in newborns is not known. The aim of this study was to measure the levels of all 3 gasotransmitters, characterise their interrelationships and elucidate their combined effects on microvascular blood flow. Methods 90 preterm neonates were studied at 24h postnatal age. Microvascular studies were performed by laser Doppler. Arterial COHb levels (a measure of CO) were determined through co-oximetry. NO was measured as nitrate and nitrite in urine. H2S was measured as thiosulphate by liquid chromatography. Relationships between levels of the gasotransmitters and microvascular blood flow were assessed through partial correlation controlling for the influence of gestational age. Structural equation modelling was used to examine the combination of these effects on microvascular blood flow and derive a theoretical model of their interactions. Results No relationship was observed between NO and CO (p = 0.18, r = 0.18). A positive relationship between NO and H2S (p = 0.008, r = 0.28) and an inverse relationship between CO and H2S (p = 0.01, r = -0.33) exists. Structural equation modelling was used to examine the combination of these effects on microvascular blood flow. The model with the best fit is presented. Conclusions The relationships between NO and H2S, and CO and H2S may be of importance in the preterm newborn, particularly as NO levels in males are associated with higher H2S levels and higher microvascular blood flow and CO in females appears to convey protection against vascular dysregulation. Here we present a theoretical model of these interactions and their overall effects on microvascular flow in the preterm newborn, upon which future mechanistic studies may be based.
引用
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页数:15
相关论文
共 77 条
[1]   Heme oxygenase-1 induction by nitric oxide in RAW 264.7 macrophages is upregulated by a cyclo-oxygenase-2 inhibitor [J].
Alcaraz, MJ ;
Habib, A ;
Créminon, C ;
Vincente, AM ;
Lebret, M ;
Lévy-Toledano, S ;
Maclouf, J .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2001, 1526 (01) :13-16
[2]   Regulation of vascular nitric oxide in vitro and in vivo; a new role for endogenous hydrogen sulphide? [J].
Ali, M. Y. ;
Ping, C. Y. ;
Mok, Y-Y P. ;
Ling, L. ;
Whiteman, M. ;
Bhatia, M. ;
Moore, P. K. .
BRITISH JOURNAL OF PHARMACOLOGY, 2006, 149 (06) :625-634
[3]   The coordination of S-sulfhydration, S-nitrosylation, and phosphorylation of endothelial nitric oxide synthase by hydrogen sulfide [J].
Altaany, Zaid ;
Ju, YoungJun ;
Yang, Guangdong ;
Wang, Rui .
SCIENCE SIGNALING, 2014, 7 (342)
[4]   Cautionary tales from the neonatal intensive care unit: Diapers may mislead urinary output estimation in extremely low birthweight infants [J].
Amey, Mark ;
Butchard, Natalie ;
Hanson, Lynne ;
Kinross, Denise ;
Mannion, Marie ;
Parsons, Justine ;
Wright, Ian M. R. .
PEDIATRIC CRITICAL CARE MEDICINE, 2008, 9 (01) :76-79
[5]   UNSUITABILITY OF CREATININE EXCRETION AS A BASIS FOR ASSESSING EXCRETION OF OTHER METABOLITES BY INFANTS AND CHILDREN [J].
APPLEGARTH, DA ;
ROSS, PM .
CLINICA CHIMICA ACTA, 1975, 64 (01) :83-85
[6]   CREATININE EXCRETION IN CHILDREN AND USEFULNESS OF CREATININE EQUIVALENTS IN AMINO ACID CHROMATOGRAPHY [J].
APPLEGARTH, DA ;
HARDWICK, DF ;
ROSS, PM .
CLINICA CHIMICA ACTA, 1968, 22 (02) :131-+
[7]   Glucocorticoid-mediated hypertension: Does the vascular smooth muscle hold all the answers? [J].
Baum, Michel ;
Moe, Orson W. .
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2008, 19 (07) :1251-1253
[8]  
Bentler P M, 1992, Stat Methods Med Res, V1, P159, DOI 10.1177/096228029200100203
[9]   Structural equation modeling in medical research: A primer [J].
Beran T.N. ;
Violato C. .
BMC Research Notes, 3 (1)
[10]   Inhibition of proteasome-mediated glucocorticoid receptor degradation restores nitric oxide bioavailability in myocardial endothelial cells in vitro [J].
Blecharz, Kinga G. ;
Burek, Malgorzata ;
Bauersachs, Johann ;
Thum, Thomas ;
Tsikas, Dimitrios ;
Widder, Julian ;
Roewer, Norbert ;
Foerster, Carola Y. .
BIOLOGY OF THE CELL, 2014, 106 (07) :219-235