The RareCyte® platform for next-generation analysis of circulating tumor cells

被引：49

作者：

Kaldjian, Eric P. ^{[1
]}

Ramirez, Arturo B. ^{[1
]}

Sun, Yao ^{[1
]}

Campton, Daniel E. ^{[1
]}

Werbin, Jeffrey L. ^{[1
]}

Varshavskaya, Paulina ^{[1
]}

Quarre, Steven ^{[1
]}

George, Tad ^{[1
]}

Madan, Anup ^{[2
]}

Blau, C. Anthony ^{[3
]}

Seubert, Ronald ^{[1
]}

机构：

[1] RareCyte Inc, 2601 4th Ave, Seattle, WA 98121 USA

[2] Covance Genom, Redmond, WA USA

[3] Univ Washington, Ctr Canc Innovat, Seattle, WA 98195 USA

来源：

CYTOMETRY PART A | 2018年 / 93A卷 / 12期

关键词：

liquid biopsy; circulating tumor cells; cell separation; immunofluorescence microscopy; molecular imaging; image processing; computer assisted; single-cell analysis; DNA amplification techniques; CTC ANALYSIS; PERIPHERAL-BLOOD; LIQUID BIOPSY; SURVIVAL; CANCER;

D O I：

10.1002/cyto.a.23619

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Circulating tumor cells (CTCs) can reliably be identified in cancer patients and are associated with clinical outcome. Next-generation "liquid biopsy" technologies will expand CTC diagnostic investigation to include phenotypic characterization and single-cell molecular analysis. We describe here a rare cell analysis platform designed to comprehensively collect and identify CTCs, enable multi-parameter assessment of individual CTCs, and retrieve single cells for molecular analysis. The platform has the following four integrated components: 1) density-based separation of the CTC-containing blood fraction and sample deposition onto microscope slides; 2) automated multiparameter fluorescence staining; 3) image scanning, analysis, and review; and 4) mechanical CTC retrieval. The open platform utilizes six fluorescence channels, of which four channels are used to identify CTC and two channels are available for investigational biomarkers; a prototype assay that allows three investigational biomarker channels has been developed. Single-cell retrieval from fixed slides is compatible with whole genome amplification methods for genomic analysis. (c) 2018 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.

引用

页码：1220 / 1225

页数：6

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