Ultra-deep two-photon fluorescence excitation in turbid media

被引：109
|
作者：
Beaurepaire, E [1 ]
Oheim, M [1 ]
Mertz, J [1 ]
机构：
[1] Ecole Super Phys & Chim Ind, INSERM, Lab Neurophysiol & Nouvelles Microscopies, EPI 0002, F-75005 Paris, France
来源：
OPTICS COMMUNICATIONS | 2001年 / 188卷 / 1-4期
关键词：
Fluorescence - Laser pulses - Optical resolving power - Photons;
D O I：
10.1016/S0030-4018(00)01156-1
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
An important application of two-photon excited fluorescence (TPEF) microscopy is to provide high-resolution images from deep within scattering media. We investigate strategies to further improve TPEF penetration depth by considering the effects of scattering on fluorescence generation and collection separately. In particular, we demonstrate that the redistribution of laser power into higher energy pulses by means of a regenerative amplifier improves the TPEF depth penetration by two to three excitation scattering mean free paths. (C) 2001 Published by Elsevier Science B.V.
引用
收藏
页码：25 / 29
页数：5
相关论文
共 50 条
  • [1] Ultra-deep penetration of temporally-focused two-photon excitation
    Sela, Gali
    Dana, Hod
    Shoham, Shy
    MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIII, 2013, 8588
  • [2] Two-photon fluorescence imaging of microspheres embedded in turbid media
    Gu, M
    Schilders, SP
    Gan, X
    JOURNAL OF MODERN OPTICS, 2000, 47 (06) : 959 - 965
  • [3] Ultra-deep brain imaging with a novel two-photon varifocal microendoscope
    Sato, Masaaki
    Motegi, Yuki
    Gengyo-Ando, Keiko
    Ohkura, Masamichi
    Nakai, Junichi
    JOURNAL OF PHARMACOLOGICAL SCIENCES, 2017, 133 (03) : S135 - S135
  • [4] Ultra-deep imaging of turbid samples by enhanced photon harvesting
    Crosignani, Viera
    Dvornikov, Alexander
    Gratton, Enrico
    MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIII, 2013, 8588
  • [5] Ultra-Deep Imaging with Cellular Resolution: Enhanced Two-Photon Fluorescence Microscopy with the Use of a Wide Area Photodetector
    Crosignani, Viera
    Dvornikov, Alexander
    Gratton, Enrico
    BIOPHYSICAL JOURNAL, 2012, 102 (03) : 199A - 199A
  • [6] Two-photon excitation fluorescence bioassays
    Hanninen, Pekka
    Soukka, Jori
    Soini, Juhani T.
    FLUORESCENCE METHODS AND APPLICATIONS: SPECTROSCOPY, IMAGING, AND PROBES, 2008, 1130 : 320 - 326
  • [7] Two-photon excitation fluorescence microscopy
    So, PTC
    Dong, CY
    Masters, BR
    Berland, KM
    ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2000, 2 : 399 - 429
  • [8] Two-photon focal modulation microscopy in turbid media
    Si, Ke
    Gong, Wei
    Chen, Nanguang
    Sheppard, Colin J. R.
    APPLIED PHYSICS LETTERS, 2011, 99 (23)
  • [9] Comparison of penetration depth between two-photon excitation and single-photon excitation in imaging through turbid tissue media
    Gu, M
    Gan, XS
    Kisteman, A
    Xu, MG
    APPLIED PHYSICS LETTERS, 2000, 77 (10) : 1551 - 1553
  • [10] Image reconstruction in tissue-like turbid media under single-photon and two-photon excitation
    Gan, XS
    Dragomir, N
    Gu, M
    MICRO- AND NANOTECHNOLOGY FOR BIOMEDICAL AND ENVIRONMENTAL APPLICATIONS, 2000, 1 : 57 - 63
12345