The Diversity of Melia azedarach L. from China Based on Transcriptome-Developed SSR Marker

Melia azedarach L. is a native tree species that can be used in a comprehensive way and is widely distributed in all provinces south of the Yellow River in China. Genetic diversity analysis of different M. azedarach germplasm sources is an important basic work for the selection, evaluation, and genetic improvement of M. azedarach germplasm resources. In this study, 100 pairs of SSR primers were designed and synthesized based on M. azedarach transcriptome data, and 16 pairs of reliable SSR primers were finally selected. The developed primers were used to analyze the genetic diversity of M. azedarach from 15 sources in 10 provinces in East, Central, and South China. The results showed that the frequency of the M. azedarach transcriptome SSR loci was high, and the distribution density was high. There were 15 sources of M. azedarach genetic diversity at a moderate level, and genetic variation was mainly present within the sources. The present study further enriches the existing SSR marker database of the M. azedarach family and can provide a reference for genetic diversity analysis and molecularly assisted breeding of M. azedarach plants at the genomic level.