Combined drought and heat stress in wheat: changes in some heat shock proteins

被引:69
作者
Grigorova, B. [1 ]
Vaseva, I. [1 ]
Demirevska, K. [1 ]
Feller, U. [2 ,3 ]
机构
[1] Bulgarian Acad Sci, Inst Plant Physiol, BG-1113 Sofia, Bulgaria
[2] Univ Bern, Inst Plant Sci, CH-3013 Bern, Switzerland
[3] Univ Bern, Oeschger Ctr Climate Change Res, CH-3013 Bern, Switzerland
基金
瑞士国家科学基金会;
关键词
electrolyte leakage; immunoblotting; relative water content; Triticum aestivum; WATER DEFICITS; ABIOTIC STRESS; ARABIDOPSIS; TEMPERATURE; CHAPERONES; RESPONSES; GROWTH;
D O I
10.1007/s10535-011-0014-x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The influence of combined and individually applied drought and heat stress was studied in two wheat (Triticum aestivum L.) cultivars: resistant cv. Katya and susceptible cv. Sadovo. Relative water content decreased and electrolyte leakage increased due to individual and combined application of both stresses. Initial heat shock protein profile has been outlined via SDS electrophoresis of leaf extracts. The results obtained were confirmed by immunoblotting with anti-HSP70 monoclonal antibodies, anti-HSP110 polyclonal antibodies and anti-alpha beta-crystalline polyclonal antibodies. The effect of simultaneously applied water stress and heat shock resembled the alterations in protein expression provoked only by water stress and differed significantly from the changes occurring after the individual application of heat stress.
引用
收藏
页码:105 / 111
页数:7
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