Modeling truncated pixel values of faint reflections in MicroED images

被引:39
作者
Hattne, Johan [1 ]
Shi, Dan [1 ]
de la Cruz, M. Jason [1 ]
Reyes, Francis E. [1 ]
Gonen, Tamir [1 ]
机构
[1] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA 20147 USA
来源
JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2016年 / 49卷
关键词
cryoEM; micro-electron diffraction; MicroED; X-ray free-electron lasers; XFELs; DATA-COLLECTION; CRYSTALLOGRAPHY; CRYSTALS; RESOLUTION;
D O I
10.1107/S1600576716007196
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The weak pixel counts surrounding the Bragg spots in a diffraction image are important for establishing a model of the background underneath the peak and estimating the reliability of the integrated intensities. Under certain circumstances, particularly with equipment not optimized for low-intensity measurements, these pixel values may be corrupted by corrections applied to the raw image. This can lead to truncation of low pixel counts, resulting in anomalies in the integrated Bragg intensities, such as systematically higher signal-to-noise ratios. A correction for this effect can be approximated by a three-parameter lognormal distribution fitted to the weakly positive-valued pixels at similar scattering angles. The procedure is validated by the improved refinement of an atomic model against structure factor amplitudes derived from corrected micro-electron diffraction (MicroED) images.
引用
收藏
页码:1029 / 1034
页数:6
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