Unequal fidelity of leading strand and lagging strand DNA replication on the Escherichia coli chromosome

被引:136
作者
Fijalkowska, IJ
Jonczyk, P
Tkaczyk, MM
Bialoskorska, M
Schaaper, RM
机构
[1] NIEHS, Mol Genet Lab, Res Triangle Pk, NC 27709 USA
[2] Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland
关键词
D O I
10.1073/pnas.95.17.10020
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We have investigated the question whether during chromosomal DNA replication in Escherichia coli the two DNA strands may be replicated with differential accuracy. This possibility of differential replication fidelity arises from the distinct modes of replication in the two strands, one strand (the leading strand) being synthesized continuously, the other (the lagging strand) discontinuously in the form of short Okazaki fragments. We have constructed a series of lacZ strains in which the lac operon is inserted into the bacterial chromosome in the two possible orientations with regard to the chromosomal replication origin oriC. Measurement of lac reversion frequencies for the two orientations, under conditions in which mutations reflect replication errors, revealed distinct differences in mutability between the two orientations. As gene inversion causes a switching of leading and lagging strands, these findings indicate that leading and lagging strand replication have differential fidelity. Analysis of the possible mispairs underlying each specific base pair substitution suggests that the lagging strand replication on the E. coli chromosome may be more accurate than leading strand replication.
引用
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页码:10020 / 10025
页数:6
相关论文
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