Needle-free, spirulina-produced Plasmodium falciparum circumsporozoite vaccination provides sterile protection against pre-erythrocytic malaria in mice

被引：8

作者：

Saveria, Tracy ^{[1
]}

Parthiban, Chaitra ^{[2
,3
]}

Seilie, Annette M. ^{[2
,3
]}

Brady, Colin ^{[1
]}

Martinez, Anissa ^{[1
]}

Manocha, Ridhima ^{[1
]}

Afreen, Esha ^{[1
]}

Zhao, Hui ^{[1
]}

Krzeszowski, Ashley ^{[1
]}

Ferrara, Jeremy ^{[1
]}

Paddock, Troy ^{[1
]}

Roberts, James ^{[1
]}

Stone, Brad C. ^{[2
,3
]}

Tasch, Michael ^{[1
]}

Murphy, Sean C. ^{[2
,3
,4
]}

机构：

[1] Lumen Biosci Inc, Seattle, WA 98109 USA

[2] Univ Washington, Dept Lab Med & Pathol, Seattle, WA 98109 USA

[3] Univ Washington, Ctr Emerging & Reemerging Infect Dis, Seattle, WA 98109 USA

[4] Univ Washington, Dept Microbiol, Seattle, WA 98109 USA

来源：

NPJ VACCINES | 2022年 / 7卷 / 01期

基金：

美国国家卫生研究院;

关键词：

B-VIRUS CORE; IMMUNE-RESPONSE; LACTOCOCCUS-LACTIS; ANTIBODY-RESPONSES; ORAL IMMUNIZATION; NASAL IMMUNIZATION; WHV INFECTION; SALMONELLA; ANTIGENS; PROTEIN;

D O I：

10.1038/s41541-022-00534-5

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Antibodies against the Plasmodium falciparum circumsporozoite protein (PfCSP) can block hepatocyte infection by sporozoites and protect against malaria. Needle-free vaccination strategies are desirable, yet most PfCSP-targeted vaccines like RTS,S require needle-based administration. Here, we evaluated the edible algae, Arthrospira platensis (commonly called 'spirulina') as a malaria vaccine platform. Spirulina were genetically engineered to express virus-like particles (VLPs) consisting of the woodchuck hepatitis B core capsid protein (WHcAg) displaying a (NANP)(15) PfCSP antigen on its surface. PfCSP-spirulina administered to mice intranasally followed by oral PfCSP-spirulina boosters resulted in a strong, systemic anti-PfCSP immune response that was protective against subcutaneous challenge with PfCSP-expressing P. yoelii. Unlike male mice, female mice did not require Montanide adjuvant to reach high antibody titers or protection. The successful use of spirulina as a vaccine delivery system warrants further development of spirulina-based vaccines as a useful tool in addressing malaria and other diseases of global health importance.

引用

页数：11

共 73 条

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