Euphausia pacifica is a good candidate for a resource of marine n-3 PUFA. However, few reports exist of the lipid and fatty acid composition of E. pacifica. To examine the potential of E. pacifica as a resource of marine n-3 PUFA, we analyzed E. pacifica oil. We extracted lipids from E. pacifica harvested from the Pacific Ocean near Sanriku, Japan. Lipid classes of E. pacifica oil were analyzed by TLC-FID and the fatty acid composition of the oil was analyzed by GC/MS. Free fatty acids and hydroxy-fatty acids were analyzed by LC/QTOFMS. The lipid content of E. pacifica ranged from 1.30% to 3.57%. The ratios of triacylglycerols, phosphatidylcholine, phosphatidylethanolamine and free fatty acids in E. pacifica lipids were 5.3-23.0%, 32.6-53.4%, 8.5-25.4% and 2.5-7.0%, respectively. The content of n-3 PUFA in E. pacifica lipids was 38.6-46.5%. We also showed that E. pacifica contains unusual fatty acids and derivatives: C16-PUFAs (9,12-hexadecadienoic acid, 6,9,12-hexadecatrienoic acid and 6,9,12,15-hexadecatetraenoic acid) and hydroxy-PUFAs (8-HETE and 10-HDoHE). E. pacifica is a good resource of marine n-3 PUFA. Moreover, E. pacifica can provide C16-PUFA and hydroxy-PUFAs.
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Lincoln Mem Univ, Coll Vet Med, Metabol Unit, 6965 Cumberland Gap Pkwy, Harrogate, TN 37752 USALincoln Mem Univ, Coll Vet Med, Metabol Unit, 6965 Cumberland Gap Pkwy, Harrogate, TN 37752 USA
Wood, Paul L.
Ball, Barry A.
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Univ Kentucky, Dept Vet Sci, Lexington, KY 40546 USALincoln Mem Univ, Coll Vet Med, Metabol Unit, 6965 Cumberland Gap Pkwy, Harrogate, TN 37752 USA
Ball, Barry A.
Scoggin, Kirsten
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Univ Kentucky, Dept Vet Sci, Lexington, KY 40546 USALincoln Mem Univ, Coll Vet Med, Metabol Unit, 6965 Cumberland Gap Pkwy, Harrogate, TN 37752 USA
Scoggin, Kirsten
Troedsson, Mats H.
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Univ Kentucky, Dept Vet Sci, Lexington, KY 40546 USALincoln Mem Univ, Coll Vet Med, Metabol Unit, 6965 Cumberland Gap Pkwy, Harrogate, TN 37752 USA
Troedsson, Mats H.
Squires, Edward L.
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Univ Kentucky, Dept Vet Sci, Lexington, KY 40546 USALincoln Mem Univ, Coll Vet Med, Metabol Unit, 6965 Cumberland Gap Pkwy, Harrogate, TN 37752 USA