Production of dengue 2 envelope domain III in plant using TMV-based vector system

被引：65
作者：
Saejung, Wanida
Fujiyama, Kazuhito
Takasaki, Tomohiko
Ito, Mikako
Hori, Koichi
Malasit, Prida
Watanabe, Yuichiro
Kurane, Ichiro
Seki, Tatsuji
机构：
[1] Osaka Univ, Int Ctr Biotechnol, Osaka 5650871, Japan
[2] Natl Inst Infect Dis, Dept Virol 1, Shinjuku Ku, Tokyo 162, Japan
[3] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, Tokyo 153, Japan
[4] Mahidol Univ, Siriraj Hosp, Fac Med, Off Res & Dev,Med Mol Biol Unit, Bangkok 10700, Thailand
来源：
VACCINE | 2007年 / 25卷 / 36期
关键词：
dengue virus serotype 2 envelope domain III protein; neutralizing antibody; tobacco mosaic virus;
D O I：
10.1016/j.vaccine.2007.06.029
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The envelope protein of dengue virus is the major protein involved in host cell receptor binding for viral entry and induction of immunity. A gene fragment encoding domain III of the dengue 2 envelope protein (D2EIII, amino acids 298-400) was successfully expressed in Nicotinana benthamiana plant using a tobacco mosaic virus (TMV)-based transient expression system. The N-terminal 5' untranslated region-omega sequence located upstream of D2EIII increased protein production in infected plant tissues. The recombinant protein was reactive with anti-D2EIII polyclonal and anti-His tag antibodies. The intramuscular immunization of mice with D2EIII induced the production of the anti-dengue virus antibody. The induced antibody demonstrated neutralizing activity against dengue type 2 virus. The result indicates that the TMV expression system produces the dengue virus antigen in plant, which possesses appropriate antigenicity and immunogenicity. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：6646 / 6654
页数：9
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