Determination of cyanocobalamin by isotope dilution LC-MS/MS

被引:17
作者
D'Ulivo, Lucia [1 ]
Yang, Lu [1 ]
Ding, Jianfu [1 ]
Pagliano, Enea [1 ]
Leek, Donald M. [1 ]
Thibeault, Marie-Pier [1 ]
Mester, Zoltan [1 ]
机构
[1] Natl Res Council Canada, 1200 Montreal Rd, Ottawa, ON K1A 0R6, Canada
关键词
Cyanocobalamin; Isotope dilution; Liquid chromatography mass spectrometry; Multivitamins; PERFORMANCE LIQUID-CHROMATOGRAPHY; COBALAMIN ANALOGS; VITAMIN-B-12; PRODUCTS; MILK; HPLC; IDENTIFICATION; DEFICIENCY; FORMULAS; TABLETS;
D O I
10.1016/j.aca.2017.07.065
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Cyanocobalamin (CNCbl) is an active form of vitamin B12, commonly employed for the preparation of multivitamin supplements and fortified food. In this study, we present a novel analytical method for its determination based on stable isotope dilution liquid chromatography electrospray tandem mass spectrometry (ID LC-MS/MS). Isotopically enriched (13)C(15)NCbl was synthesized in-house and used as internal standard. The method was validated using NIST SRM 3280 multivitamin reference material and by comparison with an independent methodology based on LC-ICPMS. The proposed method provided a detection limit of 57 pg/g and could be applied for the determination of trace level of CNCbl in multivitamin supplements with a relative standard uncertainty better than 3%. The novel ID LC-MS/MS is a primary ratio method that could become a reference for CNCbl determination in multivitamins and food supplements. The method was applied for the characterization of two NRC multivitamin tablet Certified Reference Material (CRM) candidates, VITA-1 and VITB-1 whose CNCbl levels were quantified as 2.64 +/- 0.09 and 1.75 +/- 0.12 mg/g, respectively. Crown Copyright (c) 2017 Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:103 / 109
页数:7
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