Bienzyme Sensor Based on an Oxygen Reducing Bilirubin Oxidase Electrode

被引：14
作者：
Goebel, G. [1 ]
Dietz, T. [1 ]
Lisdat, F. [1 ]
机构：
[1] Univ Appl Sci Wildau, D-15745 Wildau, Germany
来源：
ELECTROANALYSIS | 2010年 / 22卷 / 14期
关键词：
Bienzyme sensor; Bilirubin oxidase; Carbon nanotubes; Interfering substances; Biosensors; SELF-ASSEMBLED MONOLAYERS; LANGMUIR-BLODGETT-FILMS; CELLOBIOSE DEHYDROGENASE; CARBON ELECTRODES; GLUCOSE-OXIDASE; MODIFIED GOLD; ELECTROCHEMISTRY; BIOSENSORS; REDUCTION; NANOTUBES;
D O I：
10.1002/elan.200900540
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Based on an oxygen reducing electrode combining bilirubin oxidase and multiwalled carbon nanotubes modified gold (BOD-MWCNT-Au electrode) a bienzyme sensor is developed. Therefore the BOD-MWCNT-Au electrode is covalently coupled to enzymes catalysing oxygen-consuming reactions (glucose oxidase and ascorbate oxidase) to result in a membrane-free bienzyme electrode. The electrochemical characterisation of these bienzyme sensors reveals an enzyme substrate sensitivity down to 250 mu M glucose and 100 mu M ascorbate. In addition, the assembled sensor systems allow amperometric measurements in a potential range where the influence of interfering substances reacting directly at the transducing electrode is minimised. The results indicate that the BUD electrode provides a suitable platform for sensing analytes of medical and environmental interest for which oxidases of high activity are available.
引用
收藏
页码：1581 / 1585
页数：5
相关论文
共 40 条
  • [1] Electrochemical characterization of Redox polymer Langmuir-Blodgett films containing ferrocene derivatives
    Aoki, A
    Miyashita, T
    [J]. MACROMOLECULES, 1996, 29 (13) : 4662 - 4667
  • [2] Immobilization of biomaterials to nano-assembled films (self-assembled monolayers, Langmuir-Blodgett films, and layer-by-layer assemblies) and their related functions
    Ariga, Katsuhiko
    Nakanishi, Takashi
    Michinobu, Tsuyoshi
    [J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2006, 6 (08) : 2278 - 2301
  • [3] Mercaptoethylpyrazine promoted electrochemistry of redox protein and amperometric biosensing of uric acid
    Behera, S.
    Raj, C. Retna
    [J]. BIOSENSORS & BIOELECTRONICS, 2007, 23 (04) : 556 - 561
  • [4] Layer-by-layer assembly of electro-active gold nanoparticle/cytochrome c multilayers
    Bonk, Sebastian Markus
    Lisdat, Fred
    [J]. BIOSENSORS & BIOELECTRONICS, 2009, 25 (04) : 739 - 744
  • [5] A peroxidase-tetrathiafulvalene biosensor based on self-assembled monolayer modified Au electrodes for the flow-injection determination of hydrogen peroxide
    Campuzano, S
    Pedrero, M
    Pingarrón, JA
    [J]. TALANTA, 2005, 66 (05) : 1310 - 1319
  • [6] CHARACTERIZATION OF GLUCOSE OXIDASE IMMOBILIZED ON COLLAGEN
    CONSTANTINIDES, A
    VIETH, WR
    FERNANDES, PM
    [J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 1973, 1 (01) : 127 - 133
  • [7] Direct electron transfer: An approach for electrochemical biosensors with higher selectivity and sensitivity
    Freire, RS
    Pessoa, CA
    Mello, LD
    Kubota, LT
    [J]. JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY, 2003, 14 (02) : 230 - 243
  • [8] DIRECT AND INDIRECT ELECTRON-TRANSFER BETWEEN ELECTRODES AND REDOX PROTEINS
    FREW, JE
    HILL, HAO
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 172 (02): : 261 - 269
  • [9] Electrochemical investigation of cellobiose oxidation by cellobiose dehydrogenase in the presence of cytochrome c as mediator
    Fridman, V
    Wollenberger, U
    Bogdanovskaya, V
    Lisdat, F
    Ruzgas, T
    Lindgren, A
    Gorton, L
    Scheller, FW
    [J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 2000, 28 : 63 - 70
  • [10] Application of conducting polymers to biosensors
    Gerard, M
    Chaubey, A
    Malhotra, BD
    [J]. BIOSENSORS & BIOELECTRONICS, 2002, 17 (05) : 345 - 359
1234