Riboswitches: From living biosensors to novel targets of antibiotics

被引:67
作者
Aghdam, Elnaz Mehdizadeh [1 ,2 ,3 ]
Hejazi, Mohammad Saeid [1 ,2 ]
Barzegar, Abolfazl [4 ,5 ]
机构
[1] Tabriz Univ Med Sci, Fac Pharm, Dept Pharmaceut Biotechnol, Tabriz, Iran
[2] Tabriz Univ Med Sci, Mol Med Res Ctr, Tabriz, Iran
[3] Tabriz Univ Med Sci, Student Res Comm, Tabriz, Iran
[4] Univ Tabriz, RIFS, Tabriz, Iran
[5] Tabriz Univ Med Sci, SABS, Tabriz, Iran
关键词
Riboswitch; Engineered riboswitches; Biosensor; RNA; Antibacterials; Drug design; TRANSCRIPTION TERMINATION CONTROL; CONTROLS GENE-EXPRESSION; CYCLIC DI-GMP; METABOLITE-BINDING RIBOSWITCHES; CANDIDATE STRUCTURED RNAS; GRAM-POSITIVE BACTERIA; HIGH-THROUGHPUT SCREEN; IN-VITRO SELECTION; SYNTHETIC RIBOSWITCHES; S-ADENOSYLMETHIONINE;
D O I
10.1016/j.gene.2016.07.035
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Riboswitches are generally located in 5'-UTR region of mRNAs and specifically bind small ligands. Following ligand binding, gene expression is controlled mostly by transcription termination, translation inhibition or mRNA degradation processes. More than 30 classes of known riboswitches have been identified by now. Most riboswitches consist of an aptamer domain and an expression platform. The aptamer domain of each class of riboswitch is a conserved structure and stabilizes specific structures of the expression platforms through binding to specific compounds. In this review, we are highlighting most aspects of riboswitch research including the novel riboswitch discoveries, routine methods for discovering and investigating riboswitches along with newly discovered classes and mechanistic principles of riboswitch-mediated gene expression control. Moreover, we will give an overview about the potential of riboswitches as therapeutic targets for antibiotic design and also their utilization as biosensors for molecular detection. (C) 2016 Published by Elsevier B.V.
引用
收藏
页码:244 / 259
页数:16
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