Rapid and sensitive detection of norovirus genomes in oysters by a two-step isothermal amplification assay system combining nucleic acid sequence-based amplification and reverse transcription-loop-mediated isothermal amplification assays

被引：21
作者：
Fukuda, Shinji [1 ]
Sasaki, Yukie [1 ]
Seno, Masato [1 ]
机构：
[1] Hiroshima Prefectural Technol Res Inst, Ctr Publ Hlth & Environm, Minami Ku, Hiroshima 7340007, Japan
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 12期
关键词：
D O I：
10.1128/AEM.00127-08
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3 h.
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页码：3912 / 3914
页数：3
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