Synthesis, characterizations of dye-doped silica nanoparticles and their application in labeling cells

被引：31

作者：

Zhang, Nianchun ^{[1
]}

Ding, Enyong ^{[1
]}

Feng, Xue ^{[1
]}

Xu, Yang ^{[1
]}

Cai, Huaihong ^{[1
]}

机构：

[1] S China Univ Technol, Coll Mat Sci & Engn, Guangzhou 510641, Guangdong, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2012年 / 89卷

关键词：

FITC; Mesoporous materials; Nano-composite; Non-small cell lung cancer; Optical imaging; Cell uptake; FLUORESCENCE; PARTICLES;

D O I：

10.1016/j.colsurfb.2011.09.017

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Spherical silica nanoparticles coated with fluorescein isothiocyanate (FITC) dyes were prepared by using an improved Stober method. The nanoparticles were similar to 55 nm in diameter and were essentially mono-dispersed in aqueous solutions. The fluorescence excitation and emission spectra of the nano-composite particles showed no obvious shift compared with that of the free FITC in the aqueous solution. The photostability of FITC doped SiO2 nanoparticles (FDSN) increased about three-fold, compared to the free FITC in the solution. The fluorescence of FDSN was tested as imaging tools using a confocal laser scanning microscope (CLSM). From the non-small cell lung cancer (NSCLC) uptake of FDSN, it was concluded that the FDSN were incorporated into these cells. The cytotoxicity assay and the NSCLC uptake of FDSN over time were also investigated in this work. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.

引用

页码：133 / 138

页数：6

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