Cleavable ester-linked magnetic nanoparticles for labeling of solvent-exposed primary amine groups of peptides/proteins

被引：1

作者：

Patil, Ujwal S. ^{[1
]}

Osorno, Laura ^{[1
]}

Ellender, Angela ^{[1
]}

Grimm, Casey ^{[2
]}

Tarr, Matthew A. ^{[1
]}

机构：

[1] Univ New Orleans, Dept Chem, New Orleans, LA 70148 USA

[2] So Reg Res Ctr, New Orleans, LA 70124 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2015年 / 484卷

基金：

美国国家科学基金会;

关键词：

Ethylene glycol bis(succinimidylsuccinate); Core shell magnetic nanoparticles; Solvent-exposed lysine residues; SAMPLE PREPARATION; IMMOBILIZATION;

D O I：

10.1016/j.ab.2015.05.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To study the solvent-exposed lysine residues of peptides/proteins, we previously reported disulfide-linked N-hydroxysuccinimide ester-modified silica-coated iron oxide magnetic nanoparticles (NHS-SS-SiO2@Fe3O4 MNPs). The presence of a disulfide bond in the linker limits the use of disulfide reducing agent during protein digestion and allows unwanted disulfide formation between the MNPs and protein. In the current work, the disulfide bond was replaced with a cleavable ester group to synthesize NHS ester-modified SiO2@Fe3O4 MNPs. Use of the cleavable ester group provides an improved method for protein labeling and allows the use of disulfide reducing agents during protein digestion. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：18 / 20

页数：3

共 5 条

[1] Cleavable ester linked magnetic nanoparticles for labeling of solvent exposed primary amine groups of peptides/proteins
Patil, Ujwal S.
Osorno, Laura
Ellender, Angela
Grimm, Casey
Tarr, Matthew A.
DATA IN BRIEF, 2015, 4 : 302 - 307
[2] Cleavable ethylene glycol linked NHS ester modified Fe3O4@SiO2 nanoparticles for covalent labeling of primary amine groups of peptides
Osorno, Laura L.
Patil, Ujwal
Pirani, Parisa
Cai, Yang L.
Tarr, Matthew A.
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2014, 247
[3] Labeling primary amine groups in peptides/proteins using N-hydroxysuccinimidyl (NHS) ester modified superparamagnetic iron oxide nanoparticles
Patil, Ujwal S.
Tarr, Matthew A.
Cai, Yang
Qu, Haiou
O'connor, Charles J.
Sharma, Arjun
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2013, 245
[4] Labeling Primary Amine Groups in Peptides and Proteins with N-Hydroxysuccinimidyl Ester Modified Fe3O4@SiO2 Nanoparticles Containing Cleavable Disulfide-Bond Linkers
Patil, Ujwal S.
Qu, Haiou
Caruntu, Daniela
O'Connor, Charles J.
Sharma, Arjun
Cai, Yang
Tarr, Matthew A.
BIOCONJUGATE CHEMISTRY, 2013, 24 (09) : 1562 - 1569
[5] Covalent labeling of carboxylic acids and primary amine groups of peptides/proteins using functionalized Fe3O4@SiO2 nanoparticles with cleavable linkers
Patil, Ujwal S.
Cai, Yang
Tarr, Matthew A.
Pirani, Parisa
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2014, 247

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