High-Fat Diet Augments the Effect of Alcohol on Skeletal Muscle Mitochondrial Dysfunction in Mice

被引：18

作者：

Ismaeel, Ahmed ^{[1
]}

Laudato, Joseph A. ^{[2
]}

Fletcher, Emma ^{[1
]}

Papoutsi, Evlampia ^{[1
]}

Tice, Abigail ^{[2
]}

Hwa, Lara S. ^{[3
]}

Miserlis, Dimitrios ^{[4
]}

Jamurtas, Athanasios Z. ^{[5
,6
]}

Steiner, Jennifer ^{[2
]}

Koutakis, Panagiotis ^{[1
]}

机构：

[1] Baylor Univ, Dept Biol, Waco, TX 76798 USA

[2] Florida State Univ, Dept Nutr & Integrat Physiol, Tallahassee, FL 32304 USA

[3] Baylor Univ, Dept Psychol & Neurosci, Waco, TX 76798 USA

[4] Univ Texas Hlth Sci Ctr San Antonio, Dept Surg, San Antonio, TX 78229 USA

[5] Univ Thessaly, Dept Phys Educ & Sport Sci, Trikala 42100, Greece

[6] Univ Thessaly, Dept Nutr & Dietet, Trikala 42100, Greece

来源：

NUTRIENTS | 2022年 / 14卷 / 05期

基金：

美国国家卫生研究院;

关键词：

alcohol; mitochondrial function; oxidative stress; aldehyde dehydrogenase 2; CARDIAC DEPRESSION; INSULIN-RESISTANCE; OXIDATIVE STRESS; LIVER-INJURY; MOUSE MODEL; CELL-DEATH; DEHYDROGENASE; MYOPATHY; OVEREXPRESSION; MECHANISMS;

D O I：

10.3390/nu14051016

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Previous studies have shown that chronic heavy alcohol consumption and consumption of a high-fat (HF) diet can independently contribute to skeletal muscle oxidative stress and mitochondrial dysfunction, yet the concurrent effect of these risk factors remains unclear. We aimed to assess the effect of alcohol and different dietary compositions on mitochondrial activity and oxidative stress markers. Male and female mice were randomized to an alcohol (EtOH)-free HF diet, a HF + EtOH diet, or a low-Fat (LF) + EtOH diet for 6 weeks. At the end of the study, electron transport chain complex activity and expression as well as antioxidant activity and expression, were measured in skeletal muscles. Complex I and III activity were diminished in muscles of mice fed a HF + EtOH diet relative to the EtOH-free HF diet. Lipid peroxidation was elevated, and antioxidant activity was diminished, in muscles of mice fed a HF + EtOH diet as well. Consumption of a HF diet may exacerbate the negative effects of alcohol on skeletal muscle mitochondrial health and oxidative stress.

引用

页数：14

共 67 条

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