Rapid and highly sensitive coxsackievirus a indirect immunofluorescence assay typing kit for enterovirus serotyping

被引：29

作者：

Lin, Tsuey-Li ^{[1
]}

Li, Yi-Syue ^{[1
]}

Huang, Chiao-Wei ^{[1
]}

Hsu, Chiu-Chu ^{[1
]}

Wu, Ho-Sheng ^{[1
]}

Tseng, Tsan-Chang ^{[1
]}

Yang, Chen-Fu ^{[1
]}

机构：

[1] Ctr Dis Control, Ctr Res & Diagnost, Dept Hlth, Taipei 115, Taiwan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 02期

关键词：

D O I：

10.1128/JCM.01114-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe the development and evaluation of an indirect immunofluorescence assay (IFA) kit for rapid and sensitive detection of coxsackievirus A2, -4, -5, -6, and -10. This IFA kit was determined to have 95.9 to 100% sensitivity and 95.8 to 97.2% specificity. It also proved to be beneficial in reducing the number of enteroviruses that are untypeable in the clinical virology laboratory.

引用

页码：785 / 788

页数：4

共 16 条

[1]

[Anonymous], 2001, Fields Virology

[2] IDENTIFICATION OF ENTEROVIRUSES BY INDIRECT IMMUNOFLUORESCENCE USING MONOCLONAL-ANTIBODIES [J].

BASTIS, D ;

SIMONET, S ;

PATTERSON, MA ;

NEILL, S .

CLINICAL AND DIAGNOSTIC VIROLOGY, 1995, 3 (01) :83-93

[3]

Fauquet C.M., 2005, Virus taxonomy: VIIIth report of the international committee on taxonomy of viruses

[4] An epidemic of enterovirus 71 infection in Taiwan [J].

Ho, MT ;

Chen, ER ;

Hsu, KH ;

Twu, SJ ;

Chen, KT ;

Tsai, SF ;

Wang, JR ;

Shih, SR .

NEW ENGLAND JOURNAL OF MEDICINE, 1999, 341 (13) :929-935

[5]

Hyypia T, 1997, J GEN VIROL, V78, P1

[6] PRODUCTION AND STANDARDIZATION OF ECHO REFERENCE ANTISERA .1. FOR 25 PROTOTYPIC ECHO VIRUSES [J].

KAMITSUKA, P ;

WENNER, HA ;

SOERGEL, ME .

AMERICAN JOURNAL OF HYGIENE, 1961, 74 (01) :7-&

[7] Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay [J].

Klespies, SL ;

Cebula, DE ;

Kelley, CL ;

Galehouse, D ;

Maurer, CC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (06) :1465-1467

[8]

MELNICK JL, 1973, B WORLD HEALTH ORGAN, V48, P381

[9] My role in the discovery and classification of the enteroviruses [J].

Melnick, JL .

ANNUAL REVIEW OF MICROBIOLOGY, 1996, 50 :1-24

[10] Sensitive, seminested PCR amplification of VP1 sequences for direct identification of all enterovirus serotypes from original clinical specimens [J].

Nix, W. Allan ;

Oberste, M. Steven ;

Pallansch, Mark A. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (08) :2698-2704

← 1 2 →