Analysis of 12 beta-lactam antibiotics in human plasma by HPLC with ultraviolet detection

被引：194

作者：

McWhinney, Brett C. ^{[1
]}

Wallis, Steven C. ^{[2
]}

Hillister, Tara ^{[1
]}

Roberts, Jason A. ^{[2
]}

Lipman, Jeffrey ^{[2
]}

Ungerer, Jacobus P. J. ^{[1
]}

机构：

[1] Pathol Queensland, Chem Pathol, Brisbane, Qld, Australia

[2] Univ Queensland, Burns Trauma & Crit Care Res Ctr, Brisbane, Qld, Australia

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2010年 / 878卷 / 22期

关键词：

HPLC; beta-Lactam; Antibiotic; Plasma; Validation; TDM; CHROMATOGRAPHIC ANALYSIS; PENICILLINS; THERAPY; SEPSIS;

D O I：

10.1016/j.jchromb.2010.05.027

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and economical high performance liquid chromatography method was developed and validated for routine analysis of 12 Penicillin, Cephalosporin and Carbapenem antibiotics in 200 mu L of human plasma. Antibiotics determined were Ceftazidime, Meropenem, Ceftriaxone, Ampicillin, Cefazolin, Ertapenem, Cephalothin, Benzylpenicillin, Flucloxacillin, Dicloxacillin, Piperacillin and Ticarcillin. There was a common sample preparation approach involving precipitation of proteins with acetonitrile and removal of lipid-soluble components by a chloroform wash. Separations were performed on a Waters X-bridge C18 column with, depending on analytes, one of three acetonitrile-phosphate buffer mobile phases. Detection was by UV at 210, 260 and 304 nm. Validation has demonstrated the method to be linear, accurate and precise. The method has been used in a pathology laboratory for therapeutic drug monitoring (TDM) of beta-lactams in critically ill patients. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.

引用

页码：2039 / 2043

页数：5

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