Triplet excited states as chiral reporters for the binding of drugs to transport proteins

被引：87

作者：

Jiménez, MC ^{[1
]}

Miranda, MA ^{[1
]}

Vayá, I ^{[1
]}

机构：

[1] Univ Politecn Valencia, Inst Tecnol Quim, Dept Quim, E-46022 Valencia, Spain

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 29期

关键词：

D O I：

10.1021/ja0514489

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The triplet excited state of flurbiprofen methyl ester (FBPMe) has been used as a chiral reporter for the two binding sites of human serum albumin (HSA). The occupation level of the binding sites has been estimated from regression analysis of the triplet decays at several [FBPMe]/[HSA] ratios. The data agree with two high affinity binding sites (I and II) that are populated to a different extent. A remarkable stereodifferentiation has been found in the drug triplet lifetimes within the protein microenvironment. Copyright © 2005 American Chemical Society.

引用

页码：10134 / 10135

页数：2

共 13 条

[1] EXCITED TRIPLET-STATES AS PROBES IN ORGANIZED SYSTEMS - AN OVERVIEW OF RECENT RESULTS [J].

BOHNE, C ;

BARRA, M ;

BOCH, R ;

ABUIN, EB ;

SCAIANO, JC .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 1992, 65 (1-2) :249-265

[2] CLINICAL PHARMACOKINETICS OF FLURBIPROFEN AND ITS ENANTIOMERS [J].

DAVIES, NM .

CLINICAL PHARMACOKINETICS, 1995, 28 (02) :100-114

[3] The three recombinant domains of human serum albumin -: Structural characterization and ligand binding properties [J].

Dockal, M ;

Carter, DC ;

Rüker, F .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (41) :29303-29310

[4]

Evans AM, 1996, J CLIN PHARMACOL, V36, pS7

[5]

Fehske K. J., 1981, PROGR DRUG PROTEIN B, P5

[6] ATOMIC-STRUCTURE AND CHEMISTRY OF HUMAN SERUM-ALBUMIN [J].

HE, XM ;

CARTER, DC .

NATURE, 1992, 358 (6383) :209-215

[7]

JIA Z, 2002, J PHARM BIOMED ANAL, V405, P413

[8] Characterisation of the lowest singlet and triplet excited states of S-flurbiprofen [J].

Jiménez, MC ;

Miranda, MA ;

Tormos, R ;

Vayá, I .

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2004, 3 (11-12) :1038-1041

[9] PROTEIN-BINDING AND STEREOSELECTIVITY OF NONSTEROIDAL ANTIINFLAMMATORY DRUGS [J].

LAPICQUE, F ;

MULLER, N ;

PAYAN, E ;

DUBOIS, N ;

NETTER, P .

CLINICAL PHARMACOKINETICS, 1993, 25 (02) :115-125

[10] Human serum albumin-mediated stereodifferentiation in the triplet state behavior of (S)- and (R)-Carprofen [J].

Lhiaubet-Vallet, V ;

Sarabia, Z ;

Boscá, F ;

Miranda, MA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (31) :9538-9539

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