Assessment of asthmatic inflammation using hybrid fluorescence molecular tomography-x-ray computed tomography

被引：8

作者：

Ma, Xiaopeng ^{[1
,2
]}

Prakash, Jaya ^{[1
,2
]}

Ruscitti, Francesca ^{[3
]}

Glasl, Sarah ^{[1
,2
]}

Stellari, Fabio Franco ^{[4
]}

Villetti, Gino ^{[4
]}

Ntziachristos, Vasilis ^{[1
,2
]}

机构：

[1] Helmholtz Zentrum Munchen, Inst Biol & Med Imaging, Ingolstaedter Landstr 1, D-85746 Neuherberg, Germany

[2] Tech Univ Munich, Chair Biol Imaging, Ismaninger St 22, D-81675 Munich, Germany

[3] Univ Parma, Dept Biomed Biotechnol & Translat Sci, Via Taglio 10, I-43126 Parma, Italy

[4] Chiesi Farmaceutici SpA, Corp Preclin R&D, Largo Francesco Belloli 11-A, I-43122 Parma, Italy

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2016年 / 21卷 / 01期

关键词：

multimodality imaging; fluorescence molecular tomography; x-ray computed tomography; asthmatic inflammation; dust mite; ragweed; Aspergillus species-challenged mouse model; MODEL; IMMUNOLOGY; MICE; CT;

D O I：

10.1117/1.JBO.21.1.015009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear imaging plays a critical role in asthma research but is limited in its readings of biology due to the short-lived signals of radio-isotopes. We employed hybrid fluorescence molecular tomography (FMT) and x-ray computed tomography (XCT) for the assessment of asthmatic inflammation based on resolving cathepsin activity and matrix metalloproteinase activity in dust mite, ragweed, and Aspergillus species-challenged mice. The reconstructed multimodal fluorescence distribution showed good correspondence with ex vivo cryosection images and histological images, confirming FMT-XCT as an interesting alternative for asthma research. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：9

共 44 条

[1] Ale A, 2012, NAT METHODS, V9, P615, DOI [10.1038/NMETH.2014, 10.1038/nmeth.2014]
[2] Optical imaging in medicine .2. Modelling and reconstruction
Arridge, SR
Hebden, JC
[J]. PHYSICS IN MEDICINE AND BIOLOGY, 1997, 42 (05) : 841 - 853
[3] Longitudinal study of a mouse model of chronic pulmonary inflammation using breath hold gated micro-CT
Artaechevarria, Xabier
Blanco, David
Perez-Martin, Daniel
de Biurrun, Gabriel
Montuenga, Luis M.
de Torres, Juan P.
Zulueta, Javier J.
Bastarrika, Gorka
Munoz-Barrutia, Arrate
Ortiz-de-Solorzano, Carlos
[J]. EUROPEAN RADIOLOGY, 2010, 20 (11) : 2600 - 2608
[4] Matrix metalloproteinase-9 in lung remodeling
Atkinson, JJ
Senior, RM
[J]. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2003, 28 (01) : 12 - 24
[5] MRI-guided optical tomography: Prospects and computation for a new imaging method
Barbour, RL
Graber, HL
Chang, JW
Barbour, SLS
Koo, PC
Aronson, R
[J]. IEEE COMPUTATIONAL SCIENCE & ENGINEERING, 1995, 2 (04): : 63 - 77
[6] Immunology of asthma and chronic obstructive pulmonary disease
Barnes, Peter J.
[J]. NATURE REVIEWS IMMUNOLOGY, 2008, 8 (03) : 183 - 192
[7] Airway remodelling in asthma: From benchside to clinical practice
Bergeron, Celine
Tulic, Meri K.
Hamid, Qutayba
[J]. CANADIAN RESPIRATORY JOURNAL, 2010, 17 (04) : E85 - E93
[8] Advances in immunology - Asthma
Busse, WW
Lemanske, RF
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 2001, 344 (05) : 350 - 362
[9] Cherry S R, 2001, ILAR J, V42, P219
[10] Real-time assessment of inflammation and treatment response in a mouse model of allergic airway inflammation
Cortez-Retamozo, Virna
Swirski, Filip K.
Waterman, Peter
Yuan, Hushan
Figueiredo, Jose Luiz
Newton, Andita P.
Upadhyay, Rabi
Vinegoni, Claudio
Kohler, Rainer
Blois, Joseph
Smith, Adam
Nahrendorf, Matthias
Josephson, Lee
Weissleder, Ralph
Pittet, Mikael J.
[J]. JOURNAL OF CLINICAL INVESTIGATION, 2008, 118 (12) : 4058 - 4066

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