Assessment of asthmatic inflammation using hybrid fluorescence molecular tomography-x-ray computed tomography

被引：9

作者：

Ma, Xiaopeng ^{[1
,2
]}

Prakash, Jaya ^{[1
,2
]}

Ruscitti, Francesca ^{[3
]}

Glasl, Sarah ^{[1
,2
]}

Stellari, Fabio Franco ^{[4
]}

Villetti, Gino ^{[4
]}

Ntziachristos, Vasilis ^{[1
,2
]}

机构：

[1] Helmholtz Zentrum Munchen, Inst Biol & Med Imaging, Ingolstaedter Landstr 1, D-85746 Neuherberg, Germany

[2] Tech Univ Munich, Chair Biol Imaging, Ismaninger St 22, D-81675 Munich, Germany

[3] Univ Parma, Dept Biomed Biotechnol & Translat Sci, Via Taglio 10, I-43126 Parma, Italy

[4] Chiesi Farmaceutici SpA, Corp Preclin R&D, Largo Francesco Belloli 11-A, I-43122 Parma, Italy

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2016年 / 21卷 / 01期

关键词：

multimodality imaging; fluorescence molecular tomography; x-ray computed tomography; asthmatic inflammation; dust mite; ragweed; Aspergillus species-challenged mouse model; MODEL; IMMUNOLOGY; MICE; CT;

D O I：

10.1117/1.JBO.21.1.015009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear imaging plays a critical role in asthma research but is limited in its readings of biology due to the short-lived signals of radio-isotopes. We employed hybrid fluorescence molecular tomography (FMT) and x-ray computed tomography (XCT) for the assessment of asthmatic inflammation based on resolving cathepsin activity and matrix metalloproteinase activity in dust mite, ragweed, and Aspergillus species-challenged mice. The reconstructed multimodal fluorescence distribution showed good correspondence with ex vivo cryosection images and histological images, confirming FMT-XCT as an interesting alternative for asthma research. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)

引用

页数：9

共 44 条

[1]

Ale A, 2012, NAT METHODS, V9, P615, DOI [10.1038/NMETH.2014, 10.1038/nmeth.2014]

[2] Optical imaging in medicine .2. Modelling and reconstruction [J].