Lycopene attenuates zearalenone-induced oxidative damage of piglet sertoli cells through the nuclear factor erythroid-2 related factor 2 signaling pathway

被引：39
作者：
Cao, Li [1 ]
Zhao, Jie [1 ]
Ma, Li [1 ]
Chen, Jiawen [1 ]
Xu, Jingru [1 ]
Rahman, Sajid Ur [1 ,3 ]
Feng, Shibin [1 ]
Li, Yu [1 ,2 ]
Wu, Jinjie [1 ,2 ]
Wang, Xichun [1 ,2 ]
机构：
[1] Anhui Agr Univ, Coll Anim Sci & Technol, 130 West Changjiang Rd, Hefei 230036, Peoples R China
[2] Anhui Prov Key Lab Vet Pathobiol & Dis Control, Hefei, Peoples R China
[3] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Minist Agr,Key Lab Anim Parasitol, Lab Qual & Safety Risk Assessment Anim Prod Bioha, Shanghai 200241, Peoples R China
来源：
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2021年 / 225卷
关键词：
Zearalenone; Lycopene; Sertoli cells of piglets; Nrf2 signaling pathway; Oxidative stress; APOPTOSIS; STRESS; AUTOPHAGY; CYCLE; METABOLISM; EXPRESSION; TOXICITY;
D O I：
10.1016/j.ecoenv.2021.112737
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Zearalenone (ZEA) has an estrogenic effect and often causes reproductive damage. Pigs are particularly sensitive to it. Lycopene (LYC) is a type of fat-soluble natural carotenoid that has antioxidant, anti-inflammatory, anticancer, anti-cardiovascular and detoxifying effects. In this study, piglet sertoli cells (SCs) were used as research objects to investigate the mechanism of ZEA induced damage to piglet SCs and to evaluate the protective effect of LYC on ZEA induced toxic damage to piglet SCs. The results showed that ZEA damaged the cell structure and inhibited the expression of nuclear factor erythroid-2 related factor 2 (Nrf2) in the nucleus, which down regulated the relative mRNA expression of heme oxygenase 1 (HO-1) and glutathione peroxidase 1 (GPX1) and decreased the activity of HO-1, glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD), resulting in an increase in malondialdehyde (MDA) and reactive oxygen species (ROS) content. ZEA down regulated the relative mRNA and protein expression of bcl-2 in piglet SCs, promoted cell apoptosis, and upregulated the relative mRNA and protein expression of LC3, beclin-1, and bax. After 3 h LYC-pretreatment, ZEA was added for mixed culture. The results of pretreatment with LYC showed that LYC could alleviate the cytotoxicity of ZEA to porlets SCs. Compared with ZEA group, improved the cell survival rate, promoted the expression of Nrf2 in the nucleus, upregulated the relative mRNA expression of HO-1 and GPX1, increased the activity of antioxidant enzymes, and reduced the levels of MDA and ROS. Moreover, after pretreatment with LYC, the mRNA expression of bcl-2 was upregulated, the apoptosis rate was decreased, the relative mRNA and protein expressions of LC3, beclin-1 and bax were downregulated, and autophagy was alleviated. In conclusion, LYC alleviated the oxidative damage of SCs caused by ZEA by promoting the expression of Nrf2 pathway and decreased autophagy and apoptosis.
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页数：11
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