The Impact of Kappa Casein Gene Polymorphism on Milk Components and Other Productive Performance Traits of Chinese Holstein Cattle

被引:0
作者
Hamza, A. E. [1 ,2 ]
Yang, Z. P. [1 ]
Wang, X. L. [1 ]
Chen, R. J. [1 ]
Wu, H. T. [1 ]
Ibrahim, A. I. [3 ]
机构
[1] Yangzhou Univ, Anim Sci & Technol Coll, Yangzhou, Jiangsu, Peoples R China
[2] Nyala Univ, Coll Vet Sci, Dept Anim Prod, Nyala, Sudan
[3] Sinnar Univ, Fac Agr Abu Naama, Dept Anim Prod, Abu Naama, Sudan
关键词
Kappa casein genetic variance; Milk total solids and non solids; Parity; Season of calving; PROTEIN; ASSOCIATION; BREED; LOCI;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Polymorphism of kappa casein gene (kappa-Cn) at exon 5 in 259 Chinese Holstein cattle was investigated using polymerase chain reaction-single strand conformation (PCR-SSCP) technique, to verify its effect on milk composition (fat, protein, lactose, total solids (TS) and solids not fat (SNF) content and influence of other factors such as parity and season of calving; aiming at utilizing it as a genetic aid in selection to improve the quality of production of this herd. A 218 bp fragment containing exon 1V of kappa casein gene was amplified by PCR and SSCP was applied to identify the structural gene polymorphism of kappa-Cn. General linear model (GLM) was used to analyze differences between genotypes. The results indicated that kappa-Cn genotypes significantly (P<0.05) affected fat, protein and lactose content. But it had no effect (P>0.05) on TS and SNF. However, cows with genotype TT showed higher fat and protein contents (3.89 and 3.50%, respectively) than those of genotypes CC and TC (3.62 and 3.66% for fat; 3.35 and 3.36 % for protein, respectively). Except for lactose content; parity had no effect on milk components. Also, the results disclosed that season of calving did not affect (P>0.05) milk composition. This study indicated that the kappa-Cn genetic variants may be used as a genetic aid through increasing the frequency of desired genotypes to improve the quality of production of this herd. (C) 2011 PVJ. All rights reserved
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页码:153 / 156
页数:4
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