Engineering of Klebsiella oxytoca for production of 2,3-butanediol via simultaneous utilization of sugars from a Golenkinia sp hydrolysate

被引：14

作者：

Park, Jong Hyun ^{[1
]}

Choi, Min Ah ^{[1
]}

Kim, Yong Jae ^{[1
]}

Kim, Yeu-Chun ^{[1
]}

Chang, Yong Keun ^{[1
,2
]}

Jeong, Ki Jun ^{[1
,2
,3
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Plus Program BK21, 291 Daehak Ro, Daejeon 34141, South Korea

[2] Adv Biomass R&D Ctr ABC, 291 Daehak Ro, Daejeon 34141, South Korea

[3] Korea Adv Inst Sci & Technol, Inst BioCentury KIB, 291 Daehak Ro, Daejeon 34141, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 245卷

关键词：

Klebsiella oxytoca; Golenkinia sp; Galactose permease; Microalgae hydrolysate; 2,3-Butanediol; CARBON CATABOLITE REPRESSION; HIGH-LEVEL EXPRESSION; ESCHERICHIA-COLI; PROTEIN; GENE; METHYLGLYOXAL; PROMOTER; BACTERIA; ACID; MECHANISMS;

D O I：

10.1016/j.biortech.2017.05.111

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The Klebsiella oxytoca was engineered to produce 2,3-butanediol (2,3-BDO) simultaneously utilizing glucose and galactose obtained from a Golenkinia sp. hydrolysate. For efficient uptake of galactose at a high concentration of glucose, Escherichia coli galactose permease (GalP) was introduced, and the expression of galP under a weak-strength promoter resulted in simultaneous consumption of galactose and glucose. Next, to improve the sugar consumption, a gene encoding methylglyoxal synthase (MgsA) known as an inhibitor of multisugar metabolism was deleted, and the mgsA-null mutant showed much faster consumption of both sugars than the wild-type strain did. Finally, we demonstrated that the engineered K. oxytoca could utilize sugar extracts from a Golenkinia sp. hydrolysate and successfully produces 2,3-BDO. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：1386 / 1392

页数：7

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