A single starfish Aurora kinase performs the combined functions of Aurora-A and Aurora-B in human cells

被引：23

作者：

Abe, Yusuke ^{[3
]}

Okumura, Eiichi ^{[3
]}

Hosoya, Takamitsu ^{[2
]}

Hirota, Toru ^{[1
]}

Kishimoto, Takeo ^{[3
]}

机构：

[1] Japanese Fdn Canc Res, Inst Canc, Dept Expt Pathol, Koto Ku, Tokyo 1358550, Japan

[2] Tokyo Inst Technol, Grad Sch Biosci, Dept Biol Informat, Midori Ku, Yokohama, Kanagawa 2268501, Japan

[3] Tokyo Inst Technol, Grad Sch Biosci, Lab Cell & Dev Biol, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

JOURNAL OF CELL SCIENCE | 2010年 / 123卷 / 22期

关键词：

Aurora kinase; INCENP; Mitotic kinases; Molecular evolution; TPX2; GROWTH IN-VIVO; STRONGYLOCENTROTUS-PURPURATUS; CHROMOSOMAL PASSENGERS; MITOTIC ENTRY; CYCLE CONTROL; ACTIVATION; SPINDLE; TPX2; POLO; COMPLEX;

D O I：

10.1242/jcs.076315

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Aurora, an essential mitotic kinase, is highly conserved during evolution. Most vertebrates have at least two Aurora kinases, Aurora-A and Aurora-B, which have distinct functions in the centrosome-spindle and inner centromere-midbody, respectively. However, some non-vertebrate deuterostomes have only a single Aurora. It remains to be verified whether the single Aurora performs the same functions as vertebrate Auroras A and B combined. We have isolated a cDNA of a single Aurora (ApAurora) from the echinoderm starfish, Asterina pectinifera, and show that ApAurora displays most features of both Aurora-A and Aurora-B in starfish oocytes and early embryos. Furthermore, ApAurora that is stably expressed in HeLa cells can substitute for both human Aurora-A and Aurora-B when either is reduced by RNAi. A single ApAurora thus has properties of both Aurora-A and Aurora-B in starfish eggs and HeLa cells. Together with phylogenetic analysis indicating that ApAurora forms a clade with all types of vertebrate Auroras and single Auroras of non-vertebrate deuterostomes, our observations support the idea that the single Aurora found in non-vertebrate deuterostomes represents the ancestor that gave rise to various types of vertebrate Auroras. This study thus provides functional evidence for phylogenetic considerations.

引用

页码：3978 / 3988

页数：11

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