Fluorescent resonance energy transfer-based biosensor for detecting conformational changes of Pin1

被引：2

作者：

Hidaka, Masafumi ^{[1
]}

Okabe, Emiko ^{[1
]}

Hatakeyama, Kodai ^{[1
]}

Zook, Heather ^{[1
]}

Uchida, Chiyoko ^{[2
]}

Uchida, Takafumi ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Dept Mol Cell Sci, 468-1 Aoba, Sendai, Miyagi 9800845, Japan

[2] Fukushima Univ, Dept Human Dev & Culture, Kanayagawa 1, Fukushima, Fukushima 9601296, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 505卷 / 02期

关键词：

FRET; Biosensor; PPlase; Conformation; c-Myc; Phosphorylation; PROLYL ISOMERASE PIN1; C-MYC; PROTEIN; DOMAIN; REGULATOR; P53;

D O I：

10.1016/j.bbrc.2018.09.123

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pin1, a peptidyl prolyl cis/trans isomerase (PPIase), regulates the activity and stability of various phosphorylated proteins. Pinl consists of a PPlase domain and WW domain, both of which are required for the function of Pin1. However, how the behavior of these domains changes upon binding to phosphorylated proteins has not been analyzed. We created a Fluorescent Resonance Energy Transfer (FRET)-based biosensor "CPinY", which is composed of Pin1 flanked by CFP and YFP, and analyzed the interaction between Pin1 and c-Myc. Our results indicated that the dual phosphorylation of c-Myc at Thr58 and Ser62 is essential for tight interaction with Pin1 Additionally, this interaction caused a significant conformational change in Pin1. Our CPinY biosensor also detected a novel type of inhibitor of Pin1 function. We believe that his biosensor will be a novel drug screening technology targeting Pin1. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：399 / 404

页数：6

共 33 条

[1] Pin1 promotes production of Alzheimer's amyloid from β-cleaved amyloid precursor protein [J].

Akiyama, H ;

Shin, RW ;

Uchida, C ;

Kitamoto, T ;

Uchida, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 336 (02) :521-529

[2] Comparative analysis of enzyme activities and mRNA levels of peptidyl prolyl cis/trans isomerases in various organs of wild type and Pin1-/- mice [J].

Fanghanel, Jorg ;

Akiyama, Hirotada ;

Uchida, Chiyoko ;

Uchida, Takafumi .

FEBS LETTERS, 2006, 580 (13) :3237-3245

[3] Mice lacking Pin1 develop normally, but are defective in entering cell cycle from G0 arrest [J].

Fujimori, F ;

Takahashi, K ;

Uchida, C ;

Uchida, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 265 (03) :658-663

[4] Two Pathways Mediate Interdomain Allosteric Regulation in Pin1 [J].

Guo, Jingjing ;

Pang, Xiaodong ;

Zhou, Huan-Xiang .

STRUCTURE, 2015, 23 (01) :237-247

[5] Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity [J].

Helander, Sara ;

Montecchio, Meri ;

Pilstal, Robert ;

Su, Yulong ;

Kuruvilla, Jacob ;

Elven, Malin ;

Ziauddin, Javed M. E. ;

Anandapadamanaban, Madhanagopal ;

Cristobal, Susana ;

Lundstrom, Patrik ;

Sears, Rosalie C. ;

Wallner, Bjorn ;

Sunnerhagen, Maria .

STRUCTURE, 2015, 23 (12) :2267-2279

[6]

Hidaka M., 2015, J BIOL CHEM, V291, P2260

[7] Food polyphenols targeting peptidyl prolyl cis/trans isomerase Pin1 [J].

Hidaka, Masafumi ;

Kosaka, Keita ;

Tsushima, Saori ;

Uchida, Chiyoko ;

Takahashi, Katsuhiko ;

Takahashi, Noriko ;

Tsubuki, Masayoshi ;

Hara, Yukihiko ;

Uchida, Takafumi .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 499 (03) :681-687

[8] The protein level and transcription activity of activating transcription factor 1 is regulated by prolyl isomerase Pin1 in nasopharyngeal carcinoma progression [J].

Huang, Guo-Liang ;

Liao, Dan ;

Chen, Hua ;

Lu, Yan ;

Chen, Liyong ;

Li, Huahui ;

Li, Binbin ;

Liu, Weilong ;

Ye, Caiguo ;

Li, Tong ;

Zhu, Zhu ;

Wang, Jian ;

Uchida, Takafumi ;

Zou, Ying ;

Dong, Zigang ;

He, Zhiwei .

CELL DEATH & DISEASE, 2016, 7 :e2571-e2571

[9] Non-catalytic participation of the Pin1 peptidyl-prolyl isonnerase domain in target binding [J].

Innes, Brendan T. ;

Bailey, Melanie L. ;

Brandl, Christopher J. ;

Shilton, Brian H. ;

Litchfield, David W. .

FRONTIERS IN PHYSIOLOGY, 2013, 4

[10] Peptide binding induces large scale changes in inter-domain mobility in human Pin1 [J].

Jacobs, DM ;

Saxena, K ;

Vogtherr, M ;

Bernadó, P ;

Pons, M ;

Fiebig, KM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (28) :26174-26182

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