In vivo expression of innate immunity markers in patients with mycobacterium tuberculosis infection

被引:23
作者
Constantoulakis, Pantelis [2 ]
Filiou, Eftihia [2 ]
Rovina, Nikoletta [1 ]
Chras, George [1 ]
Hamhougia, Aggeliki [3 ]
Karabela, Simona [4 ]
Sotiriou, Adamandia [1 ]
Roussos, Charis [1 ]
Poulakis, Nikolaos [5 ]
机构
[1] Natl & Kapodistrian Univ Athens, Dept Pulm, Sotiria Dist Chest Dis Hosp, Athens 11528, Greece
[2] Locus Medicus, Mol Pathol & Genet Dept, Athens, Greece
[3] Univ Athens, Dept Math, Athens, Greece
[4] Nat Ctr TB, Microbiol Dpt, Athens, Greece
[5] Sotiria Dist Chest Dis Hosp, Pulm Dept 1, Athens, Greece
关键词
TOLL-LIKE RECEPTORS; INTERFERON-GAMMA ASSAY; NF-KAPPA-B; MACROPHAGE RESPONSES; PHAGOSOME MATURATION; HOST INNATE; SP110; GENE; IFN-GAMMA; SUSCEPTIBILITY; INHIBITION;
D O I
10.1186/1471-2334-10-243
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background: Toll-like receptors (TLRs), Coronin-1 and Sp110 are essential factors for the containment of Mycobacterium tuberculosis infection. The purpose of this study was to investigate the in vivo expression of these molecules at different stages of the infection and uncover possible relationships between these markers and the state of the disease. Methods: Twenty-two patients with active tuberculosis, 15 close contacts of subjects with latent disease, 17 close contacts of subjects negative for mycobacterium antigens and 10 healthy, unrelated to patients, subjects were studied. Quantitative mRNA expression of Coronin-1, Sp110, TLRs-1,-2,-4 and -6 was analysed in total blood cells vs an endogenous house-keeping gene. Results: The mRNA expression of Coronin-1, Sp110 and TLR-2 was significantly higher in patients with active tuberculosis and subjects with latent disease compared to the uninfected ones. Positive linear correlation for the expression of those factors was only found in the infected populations. Conclusions: Our results suggest that the up-regulation of Coronin-1 and Sp110, through a pathway that also includes TLR-2 up-regulation may be involved in the process of tuberculous infection in humans. However, further studies are needed, in order to elucidate whether the selective upregulation of these factors in the infected patients could serve as a specific molecular marker of tuberculosis.
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页数:10
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