Facilitating Subtiligase-Catalyzed Peptide Ligation Reactions by Using Peptide Thioester Substrates

被引：17

作者：

Tan, Xiaohong ^{[1
,2
]}

Yang, Renliang ^{[3
]}

Liu, Chuan-Fa ^{[3
]}

机构：

[1] Carnegie Mellon Univ, Dept Chem, 4400 5th Ave, Pittsburgh, PA 15213 USA

[2] Carnegie Mellon Univ, Ctr Nucle Acids Sci & Technol, Pittsburgh, PA 15213 USA

[3] Nanyang Technol Univ, Sch Biol Sci, Div Chem Biol & Biotechnol, Singapore 637551, Singapore

来源：

ORGANIC LETTERS | 2018年 / 20卷 / 21期

关键词：

NATIVE CHEMICAL LIGATION; SOLID-PHASE SYNTHESIS; UNPROTECTED PEPTIDES; PROTEIN-SYNTHESIS; THIOACIDS; LIGASE; SCOPE;

D O I：

10.1021/acs.orglett.8b02747

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

By facilitating formation of the aryl-enzyme intermediate, peptide thioesters can largely promote subtiligase-catalyzed ligation reactions over their ester counterparts. This offers a significant addition to the repertoire of enzymatic methods for protein chemical synthesis and modification.

引用

页码：6691 / 6694

页数：4

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