Construction of cytogenetic map of Gossypium herbaceum chromosome 1 and its integration with genetic maps

被引:8
作者
Cui, Xinglei [1 ]
Liu, Fang [1 ]
Liu, Yuling [1 ]
Zhou, Zhongli [1 ]
Zhao, Yanyan [1 ]
Wang, Chunying [1 ]
Wang, Xingxing [1 ]
Cai, Xiaoyan [1 ]
Wang, Yuhong [1 ]
Meng, Fei [1 ]
Peng, Renhai [1 ,2 ]
Wang, Kunbo [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol China, Anyang 455000, Henan, Peoples R China
[2] Anyang Inst Technol, Anyang 455000, Henan, Peoples R China
来源
MOLECULAR CYTOGENETICS | 2015年 / 8卷
基金
中国国家自然科学基金;
关键词
Cotton; BAC-FISH; Cytogenetic map; IN-SITU HYBRIDIZATION; BACTERIAL ARTIFICIAL CHROMOSOMES; SIMPLE SEQUENCE REPEAT; BAC-FISH; COTTON GOSSYPIUM; PHYSICAL MAP; ALLOTETRAPLOID COTTON; ARABIDOPSIS-THALIANA; POLYPLOID FORMATION; GENOME SEQUENCE;
D O I
10.1186/s13039-015-0106-y
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Background: Cytogenetic map can provide not only information of the genome structure, but also can build a solid foundation for genetic research. With the developments of molecular and cytogenetic studies in cotton (Gossypium), the construction of cytogenetic map is becoming more and more imperative. Results: A cytogenetic map of chromosome 1 (A(1)01) of Gossypium herbaceum (A(1)) which includes 10 bacterial artificial chromosome (BAC) clones was constructed by using fluorescent in situ hybridization (FISH). Meanwhile, comparison and analysis were made for the cytogenetic map of chromosome 1 (A(1)01) of G. herbaceum with four genetic linkage maps of chromosome 1 (A(h)01) of G. hirsutum ((AD)(1)) and one genetic linkage map of chromosome 1 of (A(1)01) G. arboreum (A(2)). The 10 BAC clones were also used to be localized on G. raimondii (D-5) chromosome 1 (D(5)01), and 2 of them showed clear unique hybridized signals. Furthermore, these 2 BAC clones were also shown localized on chromosome 1 of both A sub-genome and D sub-genome of G. hirsutum. Conclusion: The comparison of the cytogenetic map with genetic linkage maps showed that most of the identified marker-tagged BAC clones appearing same orders in different maps except three markers showing different positions, which might indicate chromosomal segmental rearrangements. The positions of the 2 BAC clones which were localized on A(h)01 and D(h)01 chromosomes were almost the same as that on A(1)01 and D(5)01 chromosomes. The corresponding anchored SSR markers of these 2 BAC clones were firstly found to be localized on chromosome D(5)01 (D(h)01) as they were not seen mapped like this in any genetic map reported.
引用
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页数:10
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共 61 条
  • [1] A transgenomic cytogenetic sorghum (Sorghum propinquum) bacterial artificial chromosome fluorescence in situ hybridization map of maize (Zea mays L.) pachytene chromosome 9, evidence for regions of genome hyperexpansion
    Amarillo, F. Ina E.
    Bass, Hank W.
    [J]. GENETICS, 2007, 177 (03) : 1509 - 1526
  • [2] Predicting and testing physical locations of genetically mapped loci on tomato pachytene chromosome 1
    Chang, Song-Bin
    Anderson, Lorinda K.
    Sherman, Jamie D.
    Royer, Suzanne M.
    Stack, Stephen M.
    [J]. GENETICS, 2007, 176 (04) : 2131 - 2138
  • [3] Cheng Hua Cheng Hua, 2013, Cotton Science, V25, P227
  • [4] Cheng ZK, 2001, GENETICS, V157, P1749
  • [5] Duplicated genes evolve independently after polyploid formation in cotton
    Cronn, RC
    Small, RL
    Wendel, JF
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (25) : 14406 - 14411
  • [6] Rapid diversification of the cotton genus (Gossypium: Malvaceae) revealed by analysis of sixteen nuclear and chloroplast genes
    Cronn, RC
    Small, RL
    Haselkorn, T
    Wendel, JF
    [J]. AMERICAN JOURNAL OF BOTANY, 2002, 89 (04) : 707 - 725
  • [7] de Jong JH, 1999, TRENDS PLANT SCI, V4, P258
  • [8] High-resolution mapping of YACs and the single-copy gene Hs1pro-1 on Beta vulgaris chromosomes by multi-colour fluorescence in situ hybridization
    Desel, C
    Jung, C
    Cai, DG
    Kleine, M
    Schmidt, T
    [J]. PLANT MOLECULAR BIOLOGY, 2001, 45 (01) : 113 - 122
  • [9] Development of pachytene FISH maps for six maize chromosomes and their integration with other maize maps for insights into genome structure variation
    Figueroa, Debbie M.
    Bass, Hank W.
    [J]. CHROMOSOME RESEARCH, 2012, 20 (04) : 363 - 380
  • [10] Cytogenetic map of common bean (Phaseolus vulgaris L.)
    Fonseca, Artur
    Ferreira, Joana
    Barros dos Santos, Tiago Ribeiro
    Mosiolek, Magdalena
    Bellucci, Elisa
    Kami, James
    Gepts, Paul
    Geffroy, Valerie
    Schweizer, Dieter
    dos Santos, Karla G. B.
    Pedrosa-Harand, Andrea
    [J]. CHROMOSOME RESEARCH, 2010, 18 (04) : 487 - 502