Bacterial degradation of microcystin toxins in drinking water eliminates their toxicity

被引：62
作者：
Ho, Lionel
Gaudieux, Anne-Laure
Fanok, Stella
Newcombe, Gayle
Humpage, Andrew R.
机构：
[1] S Australian Water Corp, Cooperat Res Ctr Water Qual & Treatment, Salisbury, SA 5108, Australia
[2] S Australian Water Corp, Australian Water Qual Ctr, Salisbury, SA 5108, Australia
[3] Univ Poitiers, Ecole Super Ingn Poitiers, F-86022 Poitiers, France
来源：
TOXICON | 2007年 / 50卷 / 03期
关键词：
biodegradation; cytotoxicity; microcystin; MTT; protein phosphatase 2A (PP2A); Sphingopyxis; VERO;
D O I：
10.1016/j.toxicon.2007.03.021
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Microcystin-LR and -LA were readily biodegraded by a bacterium, Sphingpoyxis sp. LH21, in a treated reservoir water. Detection of the microcystins was conducted using high-performance liquid chromatography (HPLC), protein phosphatase 2A (PP2A) inhibition assay and a cell-based cytotoxicity assay. The HPLC results correlated well with the two assays. The decrease in cytotoxicity, coupled with the associated decrease in microcystin concentrations, indicated that no cytotoxic by-products were being generated, highlighting the applicability of biodegradation as a feasible treatment option for effective microcystin removal. (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：438 / 441
页数：4
相关论文
共 19 条
[1]   Enzymatic pathway for the bacterial degradation of the cyanobacterial cyclic peptide toxin microcystin LR [J].
Bourne, DG ;
Jones, GJ ;
Blakeley, RL ;
Jones, A ;
Negri, AP ;
Riddles, P .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (11) :4086-4094
[2]   Characterisation of a gene cluster involved in bacterial degradation of the cyanobacterial toxin microcystin LR [J].
Bourne, DG ;
Riddles, P ;
Jones, GJ ;
Smith, W ;
Blakeley, RL .
ENVIRONMENTAL TOXICOLOGY, 2001, 16 (06) :523-534
[3]   Decrease in toxicity of microcystins LA and LR in drinking water by ozonation [J].
Brooke, Samuel ;
Newcombe, Gayle ;
Nicholson, Brenton ;
Klass, Gunter .
TOXICON, 2006, 48 (08) :1054-1059
[4]  
Carmichael W. W., 1998, HDB NATURAL TOXINS, V3, P121
[5]   CYANOBACTERIA SECONDARY METABOLITES - THE CYANOTOXINS [J].
CARMICHAEL, WW .
JOURNAL OF APPLIED BACTERIOLOGY, 1992, 72 (06) :445-459
[6]   Cyanobacterial toxins: Occurrence, properties and biological significance [J].
Codd, GA .
WATER SCIENCE AND TECHNOLOGY, 1995, 32 (04) :149-156
[7]  
FANOK S, IN PRESS WATER
[8]   Isolation of Adda from microcystin-LR by microbial degradation [J].
Harada, K ;
Imanishi, S ;
Kato, H ;
Mizuno, M ;
Ito, E ;
Tsuji, K .
TOXICON, 2004, 44 (01) :107-109
[9]   Determination of cyanobacterial hepatotoxins directly in water using a protein phosphatase inhibition assay [J].
Heresztyn, T ;
Nicholson, BC .
WATER RESEARCH, 2001, 35 (13) :3049-3056
[10]   Bacterial degradation of microcystin toxins within a biologically active sand filter [J].
Ho, L ;
Meyn, T ;
Keegan, A ;
Hoefel, D ;
Saint, CP ;
Newcombe, G .
WATER RESEARCH, 2006, 40 (04) :768-774
12