Identification of Bacillus anthracis by rpoB sequence analysis and multiplex PCR

被引：61
作者：
Ko, KS
Kim, JM
Kim, JW
Jung, BY
Kim, W
Kim, IJ
Kook, YH
机构：
[1] Seoul Natl Univ, Coll Med, Dept Microbiol, Seoul 110799, South Korea
[2] Seoul Natl Univ, Coll Med, SNUMRC, Canc Res Inst,Inst Endem Dis, Seoul 110799, South Korea
[3] Seoul Natl Univ Hosp, Clin Res Inst, Seoul 110799, South Korea
[4] Natl Vet Res & Quarantine Serv, Anim Dis Res Dept, Anyang 430824, Kyunggi, South Korea
[5] Natl Vet Res & Quarantine Serv, Dept Pharmacol, Anyang 430824, Kyunggi, South Korea
[6] Chung Ang Univ, Coll Med, Dept Microbiol, Seoul 156756, South Korea
[7] Inst Med Res, Seoul 156756, South Korea
[8] Dongguk Univ, Sch Med, Dept Microbiol, Gyungju, South Korea
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 07期
关键词：
D O I：
10.1128/JCM.41.7.2908-2914.2003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Comparative sequence analysis was performed upon Bacillus anthracis and its closest relatives, B. cereus and B. thuringiensis. Portions of rpoB DNA from 10 strains of B. anthracis, 16 of B. cereus, 10 of B. thuringiensis, 1 of B. mycoides, and 1 of B. megaterium were amplified and sequenced. The determined rpoB sequences (318 bp) of the 10 B. anthracis strains, including five Korean isolates, were identical to those of Ames, Florida, Kruger B, and Western NA strains. Strains of the "B. cereus group" were separated into two subgroups, in which the B. anthracis strains formed a separate clade in the phylogenetic tree. However, B. cereus and B. thuringiensis could not be differentiated. Sequence analysis confirmed the five Korean isolates as B. anthracis. Based on the rpoB sequences determined in the present study, multiplex PCR generating either B. anthracis-specific amplicons (359 and 208 bp) or cap DNA (291 bp) in a virulence plasmid could be used for the rapid differential detection and identification of virulent B. anthracis.
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页码：2908 / 2914
页数：7
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