Relationship of the inhibition of cell migration with the structure of ginseng pectic polysaccharides

被引：46

作者：

Fan, Yuying ^{[1
]}

Cheng, Hairong ^{[1
]}

Li, Shanshan ^{[1
]}

Wang, Jia ^{[1
]}

Liu, Dan ^{[1
]}

Hao, Miao ^{[1
]}

Gao, Xiaoge ^{[1
]}

Fan, Enxue ^{[1
,2
]}

Tai, Guihua ^{[1
]}

Zhou, Yifa ^{[1
]}

机构：

[1] NE Normal Univ, Sch Life Sci, Changchun 130024, Jilin Province, Peoples R China

[2] Jilin Univ, Hosp 2, Dept Surg, Changchun 130041, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 81卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Cell migration; Cell adhesion; Cell spreading; Ginseng pectin; Homogalacturonan; Rhamnogalacturonan; 1; PANAX-GINSENG; ACIDIC POLYSACCHARIDE; HYPOGLYCEMIC ACTIVITY; ANTIDIABETES DRUGS; ADHESION; GALECTIN-3; IMMUNOMODULATOR; PROTEOGLYCAN; FIBROBLASTS; COMPONENTS;

D O I：

10.1016/j.carbpol.2010.02.028

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this paper, ginseng pectin (WGPA) was fractionated and two new fractions were characterised. Nine fractions, including seven characterised in our previous paper, were tested for their effects on cell migration. The HG-domain rich pectins caused significant inhibition on cell migration. The order of the inhibitory effects is WGPA-1-HG < -2-HG < -3-HG < -4-HG. At 0.015 mg/ml, WGPA-3-HG significantly inhibited L-929 cell migration. WGPA-3-RG and -4-RG, containing both HG- and RG-1-domains, showed a slightly stronger inhibition than the HG-domain rich fractions. WGPA-N. WGPA-1-RG and -2-RG, containing no, trace or minor HG and RG-1 domains, showed less effects on cell migration. The inhibitory effect of ginseng pectic polysaccharides was related to GalA content (HG domain) and Rha content (RG-1 domain). The effect is probably mediated through decreasing cell adhesion and cell spreading. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：340 / 347

页数：8

共 39 条

[1]

Ananthakrishnan R, 2007, INT J BIOL SCI, V3, P303

[2] Ginseng pharmacology - Multiple constituents and multiple actions [J].

Attele, AS ;

Wu, JA ;

Yuan, CS .

BIOCHEMICAL PHARMACOLOGY, 1999, 58 (11) :1685-1693

[3] Proteoglycan control of cell movement during wound healing and cancer spreading [J].

Cattaruzza, S ;

Perris, R .

MATRIX BIOLOGY, 2005, 24 (06) :400-417

[4]

Chang Yuan S, 2003, Integr Cancer Ther, V2, P13, DOI 10.1177/1534735403251167

[5] A novel carbohydrate-based therapeutic GCS-100 overcomes bortezomib resistance and enhances dexamethasone-induced apoptosis in multiple myeloma cells [J].

Chauhan, D ;

Li, GL ;

Podar, K ;

Hideshima, T ;

Neri, P ;

He, DL ;

Mitsiades, N ;

Richardson, P ;

Chang, Y ;

Schindler, J ;

Carver, B ;

Anderson, KC .

CANCER RESEARCH, 2005, 65 (18) :8350-8358

[6] Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A!Meyer [J].

Choi, Kwang-tae .

ACTA PHARMACOLOGICA SINICA, 2008, 29 (09) :1109-1118

[7] Dynamic study of the transition from hyaluronan- to integrin-mediated adhesion in chondrocytes [J].

Cohen, M ;

Kam, Z ;

Addadi, L ;

Geiger, B .

EMBO JOURNAL, 2006, 25 (02) :302-311

[8] Synergistic immunostimulating activity of pidotimod and red ginseng acidic polysaccharide against cyclophosphamide-induced immunosuppression [J].

Du, Xiao Fei ;

Jiang, Cheng Zhe ;

Wu, Chun Fu ;

Won, Eun Kyung ;

Choung, Se Young .

ARCHIVES OF PHARMACAL RESEARCH, 2008, 31 (09) :1153-1159

[9] Hyaluronan-dependent pericellular matrix [J].

Evanko, Stephen P. ;

Tammi, Markku I. ;

Tammi, Raija H. ;

Wight, Thomas N. .

ADVANCED DRUG DELIVERY REVIEWS, 2007, 59 (13) :1351-1365

[10]

FLAN SK, 2005, ARCH PHARM RES, V28, P343

← 1 2 3 4 →