DNA-free genome editing methods for targeted crop improvement

被引：40
|
作者：
Kanchiswamy, Chidananda Nagamangala [1 ]
机构：
[1] FEM, Res & Innovat Ctr, Genom & Biol Fruit Crop Dept, Via Mach 1, I-38010 San Michele All Adige, TN, Italy
来源：
PLANT CELL REPORTS | 2016年 / 35卷 / 07期
关键词：
CRISPR/Cas9; RGEN; RGEN RNPs; Genome editing; PROGRAMMABLE NUCLEASES; GUIDE-RNA; CAS9; SPECIFICITY; CRISPR-CAS9; DELIVERY; CELLS; RIBONUCLEOPROTEINS; DROSOPHILA; NICKASES;
D O I：
10.1007/s00299-016-1982-2
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Evolution of the next-generation clustered, regularly interspaced, short palindromic repeat/Cas9 (CRISPR/Cas9) genome editing tools, ribonucleoprotein (RNA)-guided endonuclease (RGEN) RNPs, is paving the way for developing DNA-free genetically edited crop plants. In this review, I discuss the various methods of RGEN RNPs tool delivery into plant cells and their limitations to adopt this technology to numerous crop plants. Furthermore, focus is given on the importance of developing DNA-free genome edited crop plants, including perennial crop plants. The possible regulation on the DNA-free, next-generation genome-edited crop plants is also highlighted.
引用
收藏
页码：1469 / 1474
页数：6
相关论文
共 50 条
  • [31] Applications and potential of genome editing in crop improvement
    Yi Zhang
    Karen Massel
    Ian D. Godwin
    Caixia Gao
    Genome Biology, 19
  • [32] Genome editing technologies and their applications in crop improvement
    Mishra, Rukmini
    Zhao, Kaijun
    PLANT BIOTECHNOLOGY REPORTS, 2018, 12 (02) : 57 - 68
  • [33] Genome editing for plant research and crop improvement
    Xiangqiang Zhan
    Yuming Lu
    Jian-Kang Zhu
    Jose Ramon Botella
    JournalofIntegrativePlantBiology, 2021, 63 (01) : 3 - 33
  • [34] Genome editing for crop improvement: Challenges and opportunities
    Abdallah, Naglaa A.
    Prakash, Channapatna S.
    McHughen, Alan G.
    GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN, 2015, 6 (04): : 183 - 205
  • [35] DNA-free genome editing in tomato protoplasts using CRISPR/Cas9 ribonucleoprotein delivery
    Kang, Ga Hui
    Kang, Beum-Chang
    Han, Jeung-Sul
    Lee, Je Min
    HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, 2024, 65 (01) : 131 - 142
  • [36] DNA-free genome editing in tomato protoplasts using CRISPR/Cas9 ribonucleoprotein delivery
    Ga Hui Kang
    Beum-Chang Kang
    Jeung-Sul Han
    Je Min Lee
    Horticulture, Environment, and Biotechnology, 2024, 65 : 131 - 142
  • [37] Highly efficient DNA-free plant genome editing using virally delivered CRISPR-Cas9
    Ma, Xiaonan
    Zhang, Xiaoyan
    Liu, Huimin
    Li, Zhenghe
    NATURE PLANTS, 2020, 6 (07) : 773 - +
  • [38] DNA-free Genome Editing with Preassembled CRISPR/Cpf1 Ribonucleoproteins in Corn, Soybean and Canola.
    Shrawat, Ashok
    Wang, Dafu
    Valentine, Michelle
    Sidorov, Vladimir
    Yang, Peizhen
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2021, 57 (SUPPL 1) : S29 - S29
  • [39] DNA-Free Genome Editing in Hexaploid Sweetpotato Directed by Preassembled CRISPR-Cas9 Ribonucleoprotein Complexes
    Adrianne, Brown
    Egnin, M.
    Bukari, F.
    Mortley, D.
    Bonsi, C.
    Idehen, O.
    Alexander, D.
    Bernard, G.
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2022, 58 (04) : 676 - 676
  • [40] Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9
    Xiaonan Ma
    Xiaoyan Zhang
    Huimin Liu
    Zhenghe Li
    Nature Plants, 2020, 6 : 773 - 779
12345