Whey milk proteomics from Schistosoma mansoni-infected mice reveals proteins involved in immunomodulation of the offspring

被引:1
作者
de Holanda, Gabriela Calixto Ribeiro [1 ]
de Lima, Iasmim Lopes [1 ]
Scapin, Sandra Mara Naressi [2 ]
Silva, Maria da Conceicao [1 ]
Sales, Iana Rafaela Fernandes [3 ]
Granjeiro, Jose Mauro [2 ]
da Silva, Roberto Afonso [1 ]
de Souza, Valdenia Maria Oliveira [1 ]
de Lima Filho, Jose Luiz [1 ]
机构
[1] Univ Fed Pernambuco, LIKA, Ave Moraes Rego 1235,Cidade Univ, BR-50670901 Recife, PE, Brazil
[2] Inst Nacl Metrol Qualidade & Tecnol INMETRO, Diretoria Metrol Aplicada Ciencias Vida DIMAV, Duque De Caxias, RJ, Brazil
[3] Ctr Univ Mauricio Nassau, Recife, PE, Brazil
关键词
Breastfeeding; Immune response; Mass spectrometry; Offspring; Proteomics; Schistosomiasis; MATERNAL SCHISTOSOMIASIS; CATHEPSIN-B; IMMUNE-RESPONSE; MAMMARY-GLAND; ANTIGEN; MOTHERS; KINASE; SUSCEPTIBILITY; INVOLUTION; PREGNANCY;
D O I
10.1007/s00436-020-06643-1
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Milk from schistosomotic mothers can modulate the immune response of their offspring. However, its characterization and potential of modulating immunity has not yet been fully elucidated. Thus, the aim of this study was to evaluate whey proteins from the milk of Schistosoma mansoni-infected mice in order to identify the fractions which can act as potential immunomodulatory tools. For this, we did a mass spectrometry (nanoUPLC-MSE) analysis to characterize the proteomic profile of milk from infected (MIM) and non-infected mice (MNIM). It was possible to identify 29 differentially expressed proteins: 15 were only found in MIM, 10 only found in MNIM, and 4 were downregulated in MIM group. Gene Ontology (GO), pathway enrichment analysis, and protein-protein interaction (PPI) analyses indicated differentially expressed proteins linked to biological processes and pathways in MIM group such as the following: fructose 1,6-biphosphate metabolic and glycolytic processes, glucose metabolism, and neutrophil degranulation pathways. The downregulated and unique proteins identified in MNIM group were involved in the positive regulation of B cell activation and receptor signaling pathway, in the innate immune response, complement activation, and phagocytosis. The present findings revealed a protein profile that may be involved in the activation and deactivation of the offspring's immune system in the long term, conferring a protective character due to the previous contact with milk from infected mothers.
引用
收藏
页码:1607 / 1617
页数:11
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