Development of competition enzyme-linked immunosorbent assay for determination of formaldehyde in serum

被引：1
|
作者：
Kondo, Fumio [1 ]
Kobayashi, Shinichi [1 ]
Saito, Hiroshi [1 ]
Oka, Hisao [1 ]
Nakazawa, Hiroyuki [2 ]
Odajima, Yasuhei [3 ]
Miyazaki, Yutaka [1 ]
机构：
[1] Aichi Prefectural Inst Publ Hlth, Dept Toxicol, Kita Ku, Aichi 4628576, Japan
[2] Hoshi Univ, Dept Analyt Chem, Shinagawa Ku, Tokyo 1428501, Japan
[3] Showa Univ, Dept Pediat, Shinagawa Ku, Tokyo 1428666, Japan
来源：
BUNSEKI KAGAKU | 2007年 / 56卷 / 12期
关键词：
formaldehyde; sick house syndrome; ELISA; serum;
D O I：
10.2116/bunsekikagaku.56.1153
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Sick-building syndrome symptoms associated with indoor air volatile organic compounds, including formaldehyde in new or newly remodeled houses, have been increasingly highlighted, and are known as "sick house syndrome" in Japan. We developed a competition enzyme-linked immunosorbent assay for measuring formaldehyde in serum. The specificity of the antiserum generated with form aldehyde-rabbit serum albumin conjugates and the application to the detection of formaldehyde in serum were evaluated. The antiserum selectively reacted with formaldehyde conjugated to rabbit, human, rat and bovine-serum albumins, but not with unconjugated albumins. The quantitative working range of formaldehyde-human serum albumin conjugates was from 0.5 to 500 ng/well.
引用
收藏
页码：1153 / 1157
页数：5
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