Altered DNA-binding specificity mutants of EKLF and Sp1 show that EKLF is an activator of the β-globin locus control region in vivo

被引：56

作者：

Gillemans, N ^{[1
]}

Tewari, R ^{[1
]}

Lindeboom, F ^{[1
]}

Rottier, R ^{[1
]}

de Wit, T ^{[1
]}

Wijgerde, M ^{[1
]}

Grosveld, F ^{[1
]}

Philipsen, S ^{[1
]}

机构：

[1] Erasmus Univ, Ctr Med Genet, Dept Cell Biol, NL-3000 DR Rotterdam, Netherlands

来源：

GENES & DEVELOPMENT | 1998年 / 12卷 / 18期

关键词：

Zinc fingers; binding site specificity; locus control region; Sp1; EKLF; transcriptional activation; globin;

D O I：

10.1101/gad.12.18.2863

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The locus control region of the beta-globin cluster contains five DNase I hypersensitive sites (5'HS1-5) required for locus activation. 5'HS3 contains six G-rich motifs that are essential for its activity. Members of a protein family, characterized by three zinc fingers highly homologous to those found in transcription factor Sp1, interact with these motifs. Because point mutagenesis cannot distinguish between family members, it is not known which protein activates 5'HS3. We show that the function of such closely related proteins can be distinguished in vivo by matching point mutations in 5'HS3 with amino acid changes in the zinc fingers of Sp1 and EKLF. Testing their activity in transgenic mice shows that EKLF is a direct activator of 5'HS3.

引用

页码：2863 / 2873

页数：11

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