Streptomyces lutosisoli sp. nov., a novel actinomycete isolated from muddy soil

A novel Gram-stain positive, spore-forming, aerobic actinomycete, designated strain NEAU-QTH3-11(T), was isolated from muddy soil collected from a stream in Qitaihe, Heilongjiang Province, northeast China and characterised using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-QTH3-11(T) belongs to the genus Streptomyces and is closely related to Streptomyces rhizosphaerihabitans NBRC 109807(T) (99.38%) and Streptomyces mirabilis JCM 4791(T) (99.03%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain formed a cluster with S. rhizosphaerihabitans NBRC 109807(T) and Streptomyces siamensis NBRC 108799(T) (98.62%). The menaquinones were identified as MK-9(H-8), MK-9(H-6) and MK-9(H-4). The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as anteiso-C-15:0, iso-C-16:0, C-16:0 and C-15:0. However, multilocus sequence analysis based on five house-keeping genes (atpD, gyrB, rpoB, recA and trpB), low DNA-DNA hybridization results and some phenotypic, physiological and biochemical properties could differentiate the strain from its close relatives in the genus Streptomyces. Therefore, strain NEAU-QTH3-11(T) is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces lutosisoli sp. nov. is proposed, with NEAU-QTH3-11(T) (=DSM 42165(T)=CGMCC 4.7198(T)) as the type strain.