Simplified methods to isolate, culture and purify olfactory ensheathing cells

被引：3
|
作者：
Lu, Zhengfeng [1 ]
Shen, Yixin [1 ]
Zhang, Peng [1 ]
Fan, Zhihai [1 ]
Dong, Qirong [1 ]
Wang, Min [2 ]
机构：
[1] Soochow Univ, Affiliated Hosp 2, Dept Orthoped, Suzhou 215004, Jiangsu Prov, Peoples R China
[2] Soochow Univ, Coll Med, Lab Stem Cells, Suzhou 215004, Jiangsu Prov, Peoples R China
来源：
NEURAL REGENERATION RESEARCH | 2010年 / 5卷 / 19期
关键词：
olfactory ensheathing cells; shearing isolation primary culture punfication; in vitro olfactory bulb; rats; SPINAL-CORD-INJURY; TRANSPLANTATION; RECOVERY; GROWTH; REPAIR; BULB;
D O I：
10.3969/j.issn.1673-5374.2010.19.010
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Conventional methods for harvesting, culturing and purifying olfactory ensheathing cells are complicated time-consuming and poorly reproducible Olfactory bulbs were detached from adult Sprague Dawley rats and olfactory ensheathing cells were isolated using shearing dispersion processes After the primary cultures reached confluence the cells were purified using a three step process The olfactory ensheathing cells attached and grew rapidly The purity of the olfactory ensheathing cells increased following the three purification steps eventually exceeding 95% These cells could be maintained for an extended period time in culture This simple inexpensive reproducible method of harvesting culturing and purifying olfactory ensheathing cells shortens the culture cycle and provides sufficient olfactory ensheathing cells of controllable purity
引用
收藏
页码：1495 / 1499
页数：5
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