Inhibition of Vibrio cholerae biofilm by AiiA enzyme produced from Bacillus spp.

被引：45
作者：
Augustine, Nimmy [1 ]
Kumar, Praveen [1 ]
Thomas, Sabu [1 ]
机构：
[1] Rajiv Gandhi Ctr Biotechnol, Dept Mol Microbiol, Cholera & Environm Microbiol Lab, Trivandrum 695014, Kerala, India
来源：
ARCHIVES OF MICROBIOLOGY | 2010年 / 192卷 / 12期
关键词：
AiiA enzyme; Biofilm; Quorum sensing; V; cholerae; QUORUM; THURINGIENSIS; LACTONASE; CEREUS; GENE; PCR; DIFFERENTIATION; BACTERIA; ENHANCE;
D O I：
10.1007/s00203-010-0633-1
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Vibrio cholerae is the causative agent of water-borne diarrheal disease, cholera. The formation of biofilm favors survival and persistence of V. cholerae in the aquatic environment and also inside the host. AHL lactonase (AiiA), a metallo-beta-lactamase produced by Bacillus spp., blocks quorum sensing in Gram-negative bacteria by hydrolyzing N-acyl-homoserine lactones (AHLs). In the present investigation, AiiA-mediated inhibition of V. cholerae biofilm was studied. Two novel alleles of aiiA-encoding genes from Bacillus spp. were expressed in E. coli, and the results demonstrated that AiiA enzyme is a potent inhibitor of V. cholerae biofilm.
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收藏
页码：1019 / 1022
页数：4
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共 19 条
  • [1] The Ti plasmid of Agrobactetium tumefaciens harbors an attM-paralogous gene, aiiB, also encoding N-acyl homoserine lactonase activity
    Carlier, A
    Uroz, S
    Smadja, B
    Fray, R
    Latour, X
    Dessaux, Y
    Faure, D
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (08) : 4989 - 4993
  • [2] PCR assay of the groEL gene for detection and differentiation of Bacillus cereus group cells
    Chang, YH
    Shangkuan, YH
    Lin, HC
    Liu, HW
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (08) : 4502 - 4510
  • [3] Disruption of quorum sensing in Vibrio harveyi by the AiiA protein of Bacillus thuringiensis
    Fangfang Bai
    Yin Han
    Jixiang Chen
    Xiao-Hua Zhang
    [J]. AQUACULTURE, 2008, 274 (01) : 36 - 40
  • [4] Transition state analogs of 5′-methylthioadenosine nucleosidase disrupt quorum sensing
    Gutierrez, Jemy A.
    Crowder, Tamara
    Rinaldo-Matthis, Agnes
    Ho, Meng-Chiao
    Almo, Steven C.
    Schramm, Vern L.
    [J]. NATURE CHEMICAL BIOLOGY, 2009, 5 (04) : 251 - 257
  • [5] Quorum sensing controls biofilm formation in Vibrio cholerae
    Hammer, BK
    Bassler, BL
    [J]. MOLECULAR MICROBIOLOGY, 2003, 50 (01) : 101 - 114
  • [6] Quorum-regulated biofilms enhance the development of conditionally viable, environmental Vibrio cholerae
    Kamruzzaman, M.
    Udden, S. M. Nashir
    Cameron, D. Ewen
    Calderwood, Stephen B.
    Nair, G. Balakrish
    Mekalanos, John J.
    Faruque, Shah M.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (04) : 1588 - 1593
  • [7] Environmental determinants of Vibrio cholerae biofilm development
    Kierek, K
    Watnick, PI
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (09) : 5079 - 5088
  • [8] Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis
    Lee, SJ
    Park, SY
    Lee, JJ
    Yum, DY
    Koo, BT
    Lee, JK
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (08) : 3919 - 3924
  • [9] Bacillus cereus, Bacillus thuringiensis and Bacillus mycoides differentiation using a PCR-RE technique
    Manzano, M
    Cocolin, L
    Cantoni, C
    Comi, G
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2003, 81 (03) : 249 - 254
  • [10] Expression and characterization of aiiA gene from Bacillus subtilis BS-1
    Pana, Jieru
    Huang, Tianeei
    Yao, Fan
    Huang, Zhipeng
    Powell, Charles A.
    Qiu, Sixin
    Guan, Xiong
    [J]. MICROBIOLOGICAL RESEARCH, 2008, 163 (06) : 711 - 716
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