Mir-483 inhibits colon cancer cell proliferation and migration by targeting TRAF1

被引：18

作者：

Niu, Zi-Yu ^{[1
]}

Li, Wen-Li ^{[1
]}

Jiang, Da-Lei ^{[1
]}

Li, Yan-Song ^{[2
]}

Xie, Xiang-Jun ^{[1
]}

机构：

[1] Qingdao Municipal Hosp, Dept Gastroenterol 1, Qingdao, Peoples R China

[2] Qingdao Municipal Hosp, Ultrasonog, Qingdao, Peoples R China

来源：

KAOHSIUNG JOURNAL OF MEDICAL SCIENCES | 2018年 / 34卷 / 09期

关键词：

miR-483; Colon cancer cells; Proliferation; Migration; TRAF1; COLORECTAL-CANCER; INVASION; BEVACIZUMAB; MICRORNA; THERAPY;

D O I：

10.1016/j.kjms.2018.04.005

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

MicroRNAs are important regulators during human growth and development. Emerging evidence indicates that microRNAs play important roles in colorectal cancer. The aim of this study is to reveal the biological function and direct target gene of miR-483 in colorectal cancer. The biological function of miR-483 on the proliferation and migration of colon cancer cells was then examined by Edu assay and transwell assay, respectively. Our findings revealed that miR-483 mimic could significantly inhibit cell proliferation and migration. The target gene of miR-483 was predicted by target scan software and identified by a dual fluorescence reporter system which showed that TRAF1 was a direct target gene of miR-483 in SW480 cell line. These data suggest that miR-483 is a colorectal cancer suppressor which could inhibit cell proliferation and migration, possibly via targeting TRAF1. The miR-483 could be a potential treatment target for colorectal cancer. Copyright (C) 2018, Kaohsiung Medical University. Published by Elsevier Taiwan LLC.

引用

页码：479 / 486

页数：8

共 24 条

[1] Critical role of bevacizumab scheduling in combination with pre-surgical chemo-radiotherapy in MRI-defined high-risk locally advanced rectal cancer: results of the branch trial [J].

Avallone, Antonio ;

Pecori, Biagio ;

Bianco, Franco ;

Aloj, Luigi ;

Tatangelo, Fabiana ;

Romano, Carmela ;

Granata, Vincenza ;

Marone, Pietro ;

Leone, Alessandra ;

Botti, Gerardo ;

Petrillo, Antonella ;

Caraco, Corradina ;

Iaffaioli, Vincenzo R. ;

Muto, Paolo ;

Romano, Giovanni ;

Comella, Pasquale ;

Budillon, Alfredo ;

Delrio, Paolo .

ONCOTARGET, 2015, 6 (30) :30394-30407

[2] Location of colon cancer (right-sided versus left-sided) as a prognostic factor and a predictor of benefit from cetuximab in NCIC CO.17 [J].

Brule, S. Y. ;

Jonker, D. J. ;

Karapetis, C. S. ;

O'Callaghan, C. J. ;

Moore, M. J. ;

Wong, R. ;

Tebbutt, N. C. ;

Underhill, Cr. ;

Yip, D. ;

Zalcberg, J. R. ;

Tu, D. ;

Goodwin, R. A. .

EUROPEAN JOURNAL OF CANCER, 2015, 51 (11) :1405-1414

[3] p53 target miR-29c-3p suppresses colon cancer cell invasion and migration through inhibition of PHLDB2 [J].

Chen, Geng ;

Zhou, Tong ;

Li, Yang ;

Yu, Zhenxiang ;

Sun, Liankun .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017, 487 (01) :90-95

[4] IGF2-derived miR-483 mediated oncofunction by suppressing DLC-1 and associated with colorectal cancer [J].

Cui, Hengmi ;

Liu, Yuan ;

Jiang, Jingrui ;

Liu, Yangyang ;

Yang, Zhe ;

Wu, Shaogen ;

Cao, Wangsen ;

Cui, Isabelle H. ;

Yu, Chenggong .

ONCOTARGET, 2016, 7 (30) :48456-48466

[5] MicroRNA Replacement Therapy for miR-145 and miR-33a Is Efficacious in a Model of Colon Carcinoma [J].

Ibrahim, Ahmed Fawzy ;

Weirauch, Ulrike ;

Thomas, Maren ;

Gruenweller, Arnold ;

Hartmann, Roland K. ;

Aigner, Achim .

CANCER RESEARCH, 2011, 71 (15) :5214-5224

[6] Crystal structure of TRAF1 TRAF domain and its implications in the TRAF1-mediated intracellular signaling pathway [J].

Kim, Chang Min ;

Choi, Jae Young ;

Bhat, Eijaz Ahmed ;

Jeong, Jae-Hee ;

Son, Young-Jin ;

Kim, Sunghwan ;

Park, Hyun Ho .

SCIENTIFIC REPORTS, 2016, 6

[7] Overexpression of miR-483-5p/3p cooperate to inhibit mouse liver fibrosis by suppressing the TGF-β stimulated HSCs in transgenic mice [J].

Li, Fuyuan ;

Ma, Ning ;

Zhao, Ruiqi ;

Wu, Guodong ;

Zhang, Yanfen ;

Qiao, Yu ;

Han, Dong ;

Xu, Ya ;

Xiang, Ying ;

Yan, Bingzhu ;

Jin, Jianfeng ;

Lv, Guixiang ;

Wang, Lei ;

Xu, Changqing ;

Gao, Xu ;

Luo, Shanshun .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2014, 18 (06) :966-974

[8] SNP at miR-483-5p-binding site in the 3′-untranslated region of the BSG gene is associated with susceptibility to esophageal cancer in a Chinese population [J].

Li, H. Y. ;

Liu, Y. C. ;

Bai, Y. H. ;

Sun, M. ;

Wang, L. ;

Zhang, X. B. ;

Cai, B. .

GENETICS AND MOLECULAR RESEARCH, 2016, 15 (02)

[9] Novel molecular screening approaches in colorectal cancer [J].

Miller, Seth ;

Steele, Scott .

JOURNAL OF SURGICAL ONCOLOGY, 2012, 105 (05) :459-467

[10] Association of the polymorphisms of TRAF1 (rs10818488) and TNFAIP3 (rs2230926) with rheumatoid arthritis and systemic lupus erythematosus and their relationship to disease activity among Egyptian patients [J].

Moaaz, Mai ;

Mohannad, Nevine .

CENTRAL EUROPEAN JOURNAL OF IMMUNOLOGY, 2016, 41 (02) :165-175

← 1 2 3 →